Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma

• Published in: Bulletin of experimental biology and medicine Vol. 158; no. 3; pp. 371 - 376
• Main Authors: Shein, S. A., Nukolova, N. V., Korchagina, A. A., Abakumova, T. O., Kiuznetsov, I. I., Abakumov, M. A., Baklaushev, V. P., Gurina, O. I., Chekhonin, V. P.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-01-2015; Springer; Springer Nature B.V
• Subjects: Animals; Antibodies, Monoclonal - administration & dosage; Antibodies, Monoclonal - therapeutic use; Biomedical and Life Sciences; Biomedicine; Brain Neoplasms - drug therapy; Cancer; Cell Biology; Cell Line, Tumor; Chemotherapy; Female; Glioma - drug therapy; Gliomas; Internal Medicine; Laboratory Medicine; Liposomes - administration & dosage; Liposomes - chemistry; Monoclonal antibodies; Pathology; Proteins; Rats; Rats, Wistar; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - antagonists & inhibitors; Vascular Endothelial Growth Factor A - immunology; vascular endothelial growth factor (VEGF); brain tumors; liposomes; target delivery; monoclonal antibodies
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-015-2765-4

The efficiency of conventional chemotherapy for aggressive tumors in the CNS remains low and new strategies for the targeted delivery of anti-tumor substances are now actively developed. Pegylated liposomes covalently conjugated with monoclonal antibodies to VEGF synthesized by us are nanoparticle characterized by narrow size distribution and high dispersion stability. Immunochemical activity of antibodies after conjugation was 70% of initial level. The anti-VEGF liposomes developed by us were highly specific for VEGF
+
tumor cells (
in vitro
and
in vivo
). Intravenous injection of VEGF-liposomes to rats with intracranial C6 glioma was followed by their specific accumulation in the malignant tissues and engulfment by glioma cells, which attested to target delivery and selective accumulation of anti-VEGF-liposomes in the brain tumor. Thus, the use of targeting molecules can significantly increase the distribution and efficiency of delivery of nanocontainers to a tumor characterized by hyperexpression of the target proteins.