Filling the Void: Proximity-Based Labeling of Proteins in Living Cells

• Published in: Trends in cell biology Vol. 26; no. 11; pp. 804 - 817
• Main Authors: Kim, Dae In, Roux, Kyle J
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2016
• Subjects: Animals; APEX; BioID; Cell Survival; Humans; Pathology; Protein Binding; Protein Engineering; Protein Interaction Mapping; Proteins - metabolism; protein–protein interactions; proteomics; proximity-dependent labeling; Staining and Labeling - methods; subcellular proteome; BioID; protein–protein interactions; subcellular proteome; proximity-dependent labeling; proteomics; APEX
• ISSN: 0962-8924; 1879-3088
• DOI: 10.1016/j.tcb.2016.09.004

There are inherent limitations with traditional methods to study protein behavior or to determine the constituency of proteins in discrete subcellular compartments. In response to these limitations, several methods have recently been developed that use proximity-dependent labeling. By fusing proteins to enzymes that generate reactive molecules, most commonly biotin, proximate proteins are covalently labeled to enable their isolation and identification. In this review we describe current methods for proximity-dependent labeling in living cells and discuss their applications and future use in the study of protein behavior.