Endothelial colony-forming cells reduced the lung injury induced by cardiopulmonary bypass in rats

• Published in: Stem cell research & therapy Vol. 11; no. 1; pp. 1 - 12
• Main Authors: Sun, Haibin, Zhao, Xiaoqing, Tai, Qihang, Xu, Guangxiao, Ju, Yingnan, Gao, Wei
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 25-06-2020; BioMed Central; BMC
• Subjects: Alveoli; Anesthesia; Apoptosis; Bronchus; Cardiopulmonary bypass; Catheters; Cell number; Colonies; Colony-forming cells; Coronary artery bypass; Cytokines; Endothelial colony-forming cells; Endothelium; Experiments; Heart surgery; Inflammation; Laboratory animals; Lung injury; Lungs; Medical research; Membrane permeability; Microscopy; Oxidative stress; Peripheral blood; Permeability; Proteins; Rodents; Studies; Veins & arteries; Ventilation; United States > US
• ISSN: 1757-6512; 1757-6512
• DOI: 10.1186/s13287-020-01722-7

Background Cardiopulmonary bypass (CPB) results in severe lung injury via inflammation and endothelial injury. The aim of this study was to evaluate the effect of endothelial colony-forming cells (ECFCs) on lung injury in rats subjected to CPB. Methods Thirty-two rats were randomized into the sham, CPB, CPB/ECFC and CPB/ECFC/L-NIO groups. The rats in the sham group received anaesthesia, and the rats in the other groups received CPB. The rats also received PBS, ECFCs and L-NIO-pre-treated ECFCs. After 24 h of CPB, pulmonary capillary permeability, including the PaO.sub.2/FiO.sub.2 ratio, protein levels in bronchoalveolar lavage fluid (BALF) and lung tissue wet/dry weight were evaluated. The cell numbers and cytokines in BALF and peripheral blood were tested. Endothelial injury, lung histological injury and apoptosis were assessed. The oxidative stress response and apoptosis-related proteins were analysed. Results After CPB, all the data deteriorated compared with those obtained in the S group (sham vs CPB vs CPB/ECFC vs CPB/ECFC/L-NIO: histological score 1.62 [+ or -] 0.51 vs 5.37 [+ or -] 0.91 vs 3.37 [+ or -] 0.89 vs 4.37 [+ or -] 0.74; PaO.sub.2/FiO.sub.2 389 [+ or -] 12 vs 233 [+ or -] 36 vs 338 [+ or -] 28 vs 287 [+ or -] 30; wet/dry weight 3.11 [+ or -] 0.32 vs 6.71 [+ or -] 0.73 vs 4.66 [+ or -] 0.55 vs 5.52 [+ or -] 0.57; protein levels in BALF: 134 [+ or -] 22 vs 442 [+ or -] 99 vs 225 [+ or -] 41 vs 337 [+ or -] 53, all P < 0.05). Compared to the CPB treatment, ECFCs significantly improved pulmonary capillary permeability and PaO.sub.2/FiO.sub.2. Similarly, ECFCs also decreased the inflammatory cell number and pro-inflammatory factors in BALF and peripheral blood, as well as the oxidative stress response in the lung tissue. ECFCs reduced the lung histological injury score and apoptosis and regulated apoptosis-related proteins in the lung tissue. Compared with the CPB/ECFC group, all the indicators were partly reversed by the L-NIO. Conclusions ECFCs significantly reduced lung injury induced by inflammation after CPB. Keywords: Endothelial colony-forming cells, Cardiopulmonary bypass, Inflammation, Lung injury