Temporal dependence of cysteine protease activation following excitotoxic hippocampal injury

• Published in: Neuroscience Vol. 222; pp. 147 - 158
• Main Authors: Berry, J.N, Sharrett-Field, L.J, Butler, T.R, Prendergast, M.A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 11-10-2012; Elsevier
• Subjects: 2-Amino-5-phosphonovalerate - pharmacology; Animals; Biological and medical sciences; calpain; Calpain - metabolism; Cell Death - drug effects; Cell Survival - drug effects; Cell Survival - physiology; Cells, Cultured; cysteine protease; Cysteine Proteases - metabolism; Dipeptides - pharmacology; Enzyme Activation - physiology; Excitatory Amino Acid Agonists - toxicity; Excitatory Amino Acid Antagonists - pharmacology; excitotoxicity; Female; Fundamental and applied biological sciences. Psychology; Hippocampus - pathology; Immunohistochemistry; Male; N-Methylaspartate - toxicity; Nerve Degeneration - physiopathology; Neurology; Neurons - drug effects; Neurons - physiology; NMDA receptors; Organ Culture Techniques; Rats; Rats, Sprague-Dawley; Receptors, Glutamate - drug effects; synaptophysin; Synaptophysin - metabolism; Vertebrates: nervous system and sense organs; cornu ammonis; DG; DL-2-amino-5-phosphonopentanoic acid; DMSO; APV; phosphate-buffered saline; AMPAr; Ca 2; cysteine protease; endoG; calpain; FITC; dimethyl sulfoxide; analysis of variance; ANOVA; propidium iodide; NMDA; N-methyl-D-aspartate; TBI; excitotoxicity; fluorescein isothiocyanate; CA; PBS; synaptophysin; calcium; GluR; EAA; traumatic brain injury; NMDA receptors; dentate gyrus; excitatory amino acid; endonuclease G; glutamate receptor; PI; α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor; Ca2; Synaptophysin; Cysteine; Enzyme; Toxicity; Cysteine endopeptidases; Central nervous system; Calpain; Glutamate receptor; Encephalon; Peptidases; Excitotoxicity; Dependence; Hydrolases; Lesion; NMDA receptor; Hippocampus
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2012.07.033

Highlights ► Density of the synaptic vesicle protein synaptophysin increases with in vitro aging. ► Synaptophysin content was reduced in cell layers not demonstrating neuronal injury. ► Cysteine protease activation is temporally limited following excitotoxicity.