The impact of offspring and maternal obesogenic diets on adult offspring oocyte mitochondrial morphology in primordial and preantral follicles

• Published in: PloS one Vol. 19; no. 6; p. e0305912
• Main Authors: Xhonneux, Inne, Marei, Waleed F A, Meulders, Ben, Slootmans, Jens, Pintelon, Isabel, Leroy, Jo L M R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27-06-2024; Public Library of Science (PLoS)
• Subjects: Abnormalities; Animals; Biology and Life Sciences; Density; Diet; Diet, High-Fat - adverse effects; Electron microscopy; Factorial design; Female; Females; Fertility; Follicles; Folliculogenesis; Gametocytes; Male; Medicine and Health Sciences; Metabolism; Mice; Mitochondria; Mitochondria - metabolism; Mitochondria - ultrastructure; Morphology; Mothers; Obesity; Obesity - etiology; Obesity - metabolism; Obesity - pathology; Obesity, Maternal - metabolism; Offspring; Oocytes; Oocytes - metabolism; Oocytes - ultrastructure; Ovarian Follicle - metabolism; Ovarian Follicle - pathology; Ovarian Follicle - ultrastructure; Ovaries; Pregnancy; Prenatal Exposure Delayed Effects - pathology; Research and Analysis Methods; Sucrose; Transmission electron microscopy; Ultrastructure; Weaning
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0305912

Diet-induced obesity reduces oocyte quality mainly by impacting oocyte mitochondrial functions. Moreover, maternal obesity is associated with mitochondrial dysfunction in oocytes of their adult offspring. However, these effects were reported only in fully grown oocytes, mainly in the form of abnormal mitochondrial ultrastructure. It is unknown if obesogenic (OB) diets or maternal obesity already impact the primordial and preantral follicles. Considering the long duration and dynamics of folliculogenesis, determining the stage at which oocytes are affected and the extent of the damage is crucial for optimal reproductive management of obese patients and their daughters. Potential interaction between maternal and offspring diet effects are also not described, yet pivotal in our contemporary society. Therefore, here we examined the impact of OB diets on oocyte mitochondrial ultrastructure in primordial and activated preantral follicles in offspring from diet-induced obese or lean mothers. We used an outbred Swiss mouse model to increase the pathophysiological relevance to humans. Female mice were fed control or OB diets for 7 weeks, then mated with control males. Their female offspring were fed control or OB diets after weaning for 7 weeks (2-by-2 factorial design). Adult offspring ovarian sections were examined using transmission electron microscopy. We characterised and classified unique features of oocyte mitochondrial ultrastructure in the preantral follicles. An increase in mitochondrial matrix density was the most predominant change during follicle activation in secondary follicles, a feature that is linked with a higher mitochondrial activity. Maternal obesity increased mitochondrial density already in the primordial follicles suggesting an earlier increase in bioenergetic capacity. Maternal obesity did not induce abberant ultrastructure (abnormalities and defects) in primordial or preantral follicles. In contrast, offspring OB diet increased mitochondrial abnormalities in the primordial follicles. Further investigation of the consequences of these changes on oocyte metabolic regulation and stress levels during folliculogenesis is needed.