Relationship Between GHb Concentration and Erythrocyte Survival Determined From Breath Carbon Monoxide Concentration

• Published in: Diabetes care Vol. 27; no. 4; pp. 931 - 935
• Main Authors: VIRTUE, Mark A, FURNE, Julie K, NUTTALL, Frank Q, LEVITT, Michael D
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01-04-2004
• Subjects: Adult; Aged; Biological and medical sciences; Breath Tests; Carbon Monoxide; Case studies; Diabetes; Diabetes Mellitus, Type 2 - blood; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Erythrocyte Aging; Erythrocytes; Glucose; Glycated Hemoglobin A - metabolism; Humans; Hyperglycemia; Hyperglycemia - blood; Medical sciences; Middle Aged; Osmolar Concentration; Reticulocyte Count; Minnesota; Endocrinopathy; Red blood cell; Carbon monoxide; Concentration; Survival; Diabetes mellitus
• ISSN: 0149-5992; 1935-5548
• DOI: 10.2337/diacare.27.4.931

Relationship Between GHb Concentration and Erythrocyte Survival Determined From Breath Carbon Monoxide Concentration
Mark A. Virtue , MD ,
Julie K. Furne , BS ,
Frank Q. Nuttall , MD, PHD and
Michael D. Levitt , MD
From the Minneapolis Veterans Administration Medical Center, Minneapolis, Minnesota
Address correspondence and reprint requests to Michael D. Levitt, MD, ACOS for Research, Minneapolis Veterans Affairs Medical
Center (151), 1 Veterans Dr., Minneapolis, MN 55417. E-mail: michael.levitt{at}med.va.gov
Abstract
OBJECTIVE —Subjects with decreased erythrocyte survival have an unusually low GHb percentage. The goal of this study was to determine
whether hyperglycemia, as reflected by GHb percentage, is associated with decreased erythrocyte survival.
RESEARCH DESIGN AND METHODS —Erythrocyte survival was quantitated in 23 subjects with type 2 diabetes, and these values were correlated with the subjects’
GHb percentage. Erythrocyte survival was determined from the difference between the subjects’ alveolar carbon monoxide (CO)
concentration and atmospheric CO concentration. Reticulocyte counts were obtained in 16 subjects.
RESULTS —Although the vast majority of the subjects had erythrocyte life spans that fell within the normal range (123 ± 23 days),
there was a highly significant inverse correlation ( r = −0.66, P < 0.01) between life span and GHb percentage, with an average decline in life span of 6.9 days for each 1% rise in GHb. The
reticulocyte count inversely correlated with erythrocyte life span ( r = −0.77, P < 0.01).
CONCLUSIONS —Hyperglycemia, as evidenced by high GHb percentage, is associated with an appreciable decrease in erythrocyte life span.
Because GHb appears to be formed over the lifetime of the erythrocyte, this decreased erythrocyte survival suggests that high
GHb percentages may systematically underestimate the true degree of hyperglycemia.
Footnotes
See accompanying editorial, p. 1013.
Accepted December 2, 2003.
Received September 3, 2003.
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