Investigation of CD4 and CD8 T cell-mediated protection against influenza A virus in a cohort study

• Published in: BMC medicine Vol. 20; no. 1; pp. 1 - 230
• Main Authors: Tsang, Tim K, Lam, Kwok-Tai, Liu, Yinping, Fang, Vicky J, Mu, Xiaofeng, Leung, Nancy H. L, Peiris, J. S. Malik, Leung, Gabriel M, Cowling, Benjamin J, Tu, Wenwei
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 21-07-2022; BioMed Central; BMC
• Subjects: Blood; CD4 antigen; CD8 antigen; Cell-mediated immunity; Children & youth; Cohort analysis; Epidemics; Health aspects; Hemagglutination inhibition; Herd immunity; Households; Immunity; Infections; Influenza; Influenza A; Influenza vaccines; Influenza viruses; Laboratories; Lymphocytes; Lymphocytes T; Pandemics; Physiological aspects; Statistical analysis; Susceptibility; T cell-mediated immunity; T cells; Testing; Vaccines; Viruses; γ-Interferon; China; St Louis Missouri; United States > US; California
• ISSN: 1741-7015; 1741-7015
• DOI: 10.1186/s12916-022-02429-7

Background The protective effect of T cell-mediated immunity against influenza virus infections in natural settings remains unclear, especially in seasonal epidemics. Methods To explore the potential of such protection, we analyzed the blood samples collected longitudinally in a community-based study and covered the first wave of pandemic H1N1 (pH1N1), two subsequent pH1N1 epidemics, and three seasonal H3N2 influenza A epidemics (H3N2) for which we measured pre-existing influenza virus-specific CD4 and CD8 T cell responses by intracellular IFN-[gamma] staining assay for 965 whole blood samples. Results Based on logistic regression, we found that higher pre-existing influenza virus-specific CD4 and CD8 T cell responses were associated with lower infection odds for corresponding subtypes. Every fold increase in H3N2-specific CD4 and CD8 T cells was associated with 28% (95% CI 8%, 44%) and 26% (95% CI 8%, 41%) lower H3N2 infection odds, respectively. Every fold increase in pre-existing seasonal H1N1 influenza A virus (sH1N1)-specific CD4 and CD8 T cells was associated with 28% (95% CI 11%, 41%) and 22% (95% CI 8%, 33%) lower pH1N1 infection odds, respectively. We observed the same associations for individuals with pre-epidemic hemagglutination inhibition (HAI) titers < 40. There was no correlation between pre-existing influenza virus-specific CD4 and CD8 T cell response and HAI titer. Conclusions We demonstrated homosubtypic and cross-strain protection against influenza infections was associated with T cell response, especially CD4 T cell response. These protections were independent of the protection associated with HAI titer. Therefore, T cell response could be an assessment of individual and population immunity for future epidemics and pandemics, in addition to using HAI titer. Keywords: Influenza, Susceptibility, T cell-mediated immunity