Beclin-1-Dependent Autophagy of Left Ventricular Cardiomyocytes in SHR and Wistar-Kyoto Rats with Type 1 Diabetes Mellitus

• Published in: Bulletin of experimental biology and medicine Vol. 171; no. 1; pp. 23 - 27
• Main Authors: Blagonravov, M. L., Sklifasovskaya, A. P., Demurov, E. A., Karimov, A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-05-2021; Springer; Springer Nature B.V
• Subjects: Animals; Autophagy; Beclin-1 - genetics; Beclin-1 - metabolism; Biomedical and Life Sciences; Biomedicine; Cardiomyocytes; Cell Biology; Congestive heart failure; Diabetes; Diabetes mellitus (insulin dependent); Diabetes Mellitus, Type 1 - metabolism; Heart cells; Heart Ventricles; Hypertension; Internal Medicine; Laboratory Medicine; Male; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Pathology; Phagocytosis; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Streptozocin; Type 1 diabetes; Ventricle; Beclin-1; arterial hypertension; myocardium; diabetes mellitus; autophagy
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-021-05164-w

Autophagy is considered as a mechanism of progression of heart failure, but under certain conditions, it can also act as an adaptation mechanism. Beclin-1 plays the key role in autophagy. We studied the features of Beclin-1 expression in cardiomyocytes of the left ventricle in arterial hypertension, type 1 diabetes mellitus, and their combination. The experiment was performed on male 38-week-old Wistar Kyoto rats and SHR rats aged 38 and 57 weeks. Type 1 diabetes mellitus was modeled by a single parenteral administration of streptozotocin. Expression of Beclin-1 in cardiomyocytes of the left ventricle was evaluated by immunohistochemical analysis. In arterial hypertension, a decrease in the intensity of Beclin-1-dependent autophagy of cardiomyocytes was seen, while in combined pathology, we observed an opposite effect manifested as a significant increase in the expression of protein Beclin-1.