Data Base Analysis of Protein Expression Patterns During T-Cell Ontogeny and Activation

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 90; no. 8; pp. 3314 - 3318
• Main Authors: Hanash, S. M., Strahler, J. R., Chan, Y., Kuick, R., Teichroew, D., Neel, J. V., Hailat, N., Keim, D. R., Gratiot-Deans, J., Ungar, D., Melhem, R., Zhu, X. X., Andrews, P., Lottspeich, F., Eckerskorn, C., Chu, E., Ali, I., Fox, D. A., Richardson, B. C., Turka, L. A.
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15-04-1993; National Acad Sciences; National Academy of Sciences
• Subjects: 550200 - Biochemistry; activation; Amino Acid Sequence; ANIMAL CELLS; B lymphocytes; BASIC BIOLOGICAL SCIENCES; Biochemistry; Biological and medical sciences; CD4 Antigens - analysis; CD8 Antigens - analysis; Cell Differentiation; Cell growth; Child, Preschool; data base; DATA BASE MANAGEMENT; Databases, Factual; development; DISEASES; Electrophoresis, Gel, Two-Dimensional; Enzymes - genetics; expression; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gels; Genetics; Humans; IMMUNE SYSTEM DISEASES; Immunobiology; LEUKEMIA; Lymphocyte Activation; Lymphocytes; lymphocytes T; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; man; MANAGEMENT; Molecular Sequence Data; NEOPLASMS; ONTOGENESIS; ORGANIC COMPOUNDS; PEPTIDES; POLYPEPTIDES; PROTEINS; Proteins - genetics; Proteins - isolation & purification; Sequencing; SOMATIC CELLS; T lymphocytes; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - physiology; T-Lymphocytes - cytology; T-Lymphocytes - immunology; T-Lymphocytes - physiology; THYMOCYTES; Thymus Gland - immunology; Thymus Gland - physiology; Human; Lymphoid cell; Thymus gland; Gel electrophoresis; Thymocyte; Activation; Ripening; Proteins; Lymphoid organ; T-Lymphocyte; Database; Development; Two dimensional electrophoresis
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.8.3314

We have developed a data base of lymphoid proteins detectable by two-dimensional polyacrylamide gel electrophoresis. The data base contains two-dimensional patterns and derived information pertaining to polypeptide constituents of unstimulated and stimulated mature T cells and immature thymocytes, single-cell-derived T- and B-cell clones, leukemia cells, and lymphoid cell lines. Using this data base, we have compared the protein constituents of mature T cells and immature thymocytes before and after mitotic stimulation. A subset of polypeptides that are induced in mature T cells following mitotic stimulation were found to be constitutively expressed in immature thymocytes. Other polypeptides exhibited differences in their expression between mature and immature thymocytes in a manner unrelated to proliferation. The identity of several constitutively expressed or mitotically induced proteins in lymphoid cells was established by microsequencing. These initial findings point to significant differences in the molecular pathways leading to proliferation between mature and immature T cells. The construction of this database should facilitate further studies of lymphoid differentiation and function.