A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

• Published in: International journal of antimicrobial agents Vol. 37; no. 4; pp. 356 - 359
• Main Authors: Voets, Guido M, Fluit, A.C, Scharringa, Jelle, Cohen Stuart, James, Leverstein-van Hall, Maurine A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-04-2011; Elsevier
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Bacterial Proteins - metabolism; Base Sequence; beta-lactamase; beta-Lactamases - metabolism; Biological and medical sciences; Carbapenemases; carbapenems; cephalosporins; DNA Primers; Extended-spectrum β-lactamases; Genotype; Infectious Disease; Life Sciences; Medical sciences; Microbiology and Parasitology; microorganisms; Multiplex PCR; OXA β-lactamases; Pharmacology. Drug treatments; Plasmid-mediated AmpC β-lactamases; Plasmids; polymerase chain reaction; Polymerase Chain Reaction - methods; Extended-spectrum β-lactamases; Multiplex PCR; Plasmid-mediated AmpC β-lactamases; OXA β-lactamases; Carbapenemases; Plasmid; Multiplex polymerase chain reaction; Extended-spectrum β-lactamase; Genotype; Diagnosis; Detection
• ISSN: 0924-8579; 1872-7913
• DOI: 10.1016/j.ijantimicag.2011.01.005

Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.