The Role of Vimentin Intermediate Filaments in Cortical and Cytoplasmic Mechanics

• Published in: Biophysical journal Vol. 105; no. 7; pp. 1562 - 1568
• Main Authors: Guo, Ming, Ehrlicher, Allen J., Mahammad, Saleemulla, Fabich, Hilary, Jensen, Mikkel H., Moore, Jeffrey R., Fredberg, Jeffrey J., Goldman, Robert D., Weitz, David A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2013; Biophysical Society; The Biophysical Society
• Subjects: Animals; Biophysics; Cell Biophysics; Cells; Cytoplasm; Cytoplasm - metabolism; Cytoplasmic Vesicles - metabolism; Cytoskeleton; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; fibroblasts; Fibroblasts - metabolism; Fibroblasts - ultrastructure; intermediate filaments; Mechanical properties; Mice; Mice, Knockout; microfilaments; microtubules; modulus of elasticity; Optical Tweezers; organelles; Protein Transport; Rheology; Rodents; Shear Strength; vimentin; Vimentin - genetics; Vimentin - metabolism
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/j.bpj.2013.08.037

The mechanical properties of a cell determine many aspects of its behavior, and these mechanics are largely determined by the cytoskeleton. Although the contribution of actin filaments and microtubules to the mechanics of cells has been investigated in great detail, relatively little is known about the contribution of the third major cytoskeletal component, intermediate filaments (IFs). To determine the role of vimentin IF (VIF) in modulating intracellular and cortical mechanics, we carried out studies using mouse embryonic fibroblasts (mEFs) derived from wild-type or vimentin−/− mice. The VIFs contribute little to cortical stiffness but are critical for regulating intracellular mechanics. Active microrheology measurements using optical tweezers in living cells reveal that the presence of VIFs doubles the value of the cytoplasmic shear modulus to ∼10 Pa. The higher levels of cytoplasmic stiffness appear to stabilize organelles in the cell, as measured by tracking endogenous vesicle movement. These studies show that VIFs both increase the mechanical integrity of cells and localize intracellular components.