Improved production of enzymes, which are expressed under the Pho regulon promoter, in the rmf gene (encoding ribosome modulation factor) disruptant of Escherichia coli

• Published in: Bioscience, biotechnology, and biochemistry Vol. 70; no. 4; pp. 949 - 957
• Main Authors: Imaizumi, A.(Ajinomoto Co. Inc., Kawasaki, Kanagawa (Japan)), Koseki, C, Matsui, K, Kojima, H
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-04-2006; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: ACID PHOSPHATASE; acid phosphatase/nucleoside pyrophosphate phosphotransferase; ALKALINE PHOSPHATASE; Alkaline Phosphatase - metabolism; Base Sequence; Biological and medical sciences; Cell Proliferation - drug effects; Culture Media; Down-Regulation - drug effects; Down-Regulation - genetics; ESCHERICHIA COLI; Escherichia coli - cytology; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; EXPRESION GENICA; EXPRESSION DES GENES; FOSFATASA ACIDA; FOSFATASA ALCALINA; Fundamental and applied biological sciences. Psychology; GENE; GENE EXPRESSION; Gene Expression Profiling; Gene Expression Regulation, Bacterial - genetics; Gene Expression Regulation, Enzymologic - genetics; GENES; Molecular Sequence Data; Morganella morganii; PHOSPHATASE ACIDE; PHOSPHATASE ALCALINE; Phosphates - pharmacology; Promoter Regions, Genetic - genetics; Regulon - genetics; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; rmf gene; Up-Regulation - drug effects; Up-Regulation - genetics; acid phosphatase/nucleoside pyrophosphate phosphotransferase; Alkaline phosphatase; Nucleoside phosphotransferase; Ribosome; rmf gene; Enzyme; Transferases; Escherichia coli; Phosphoric monoester hydrolases; Esterases; Gene; Modulation; Production; Bacteria; Hydrolases; Acid phosphatase; Enterobacteriaceae; Morganella morganii
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.70.949

Using a DNA macroarray, we investigated the effects of rmf gene (encoding ribosome modulation factor) disruption on gene expression profiles in Escherichia coli. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, phoA, increased in the rmf disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from Morganella morganii also increased significantly in the rmf disruptant. We concluded that host modification by the rmf gene disruption has potential benefit in industrial enzyme production using Escherichia coli.