Resequencing microarray for detection of human adenoviruses in patients with conjunctivitis

• Published in: Journal of clinical virology Vol. 47; no. 3; pp. 282 - 285
• Main Authors: Woo, Patrick C.Y, Lau, Susanna K.P, Choi, Garnet K.Y, Fung, Hin-Tat, Shek, Kam-Chuen, Miao, Ji, Chan, Benedict Y.L, Ng, Kenneth H.L, Ngan, Antonio H.Y, Ellis-Behnke, Rutledge, Que, Tak-Lun, Kam, Chak-Wah, Yuen, Kwok-Yung
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-03-2010; Elsevier
• Subjects: Adenovirus Infections, Human - diagnosis; Adenovirus Infections, Human - virology; Adenoviruses, Human - isolation & purification; Adolescent; Adult; Aged; Aged, 80 and over; Allergy and Immunology; Biological and medical sciences; Child; Conjunctivitis; Conjunctivitis - virology; Culture; Female; Fundamental and applied biological sciences. Psychology; Human adenovirus; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Male; Medical sciences; Microarray Analysis - methods; Microbiology; Middle Aged; Miscellaneous; Patients; PCR; Prospective Studies; Resequencing microarray; Sensitivity and Specificity; Viral diseases; Virology; Young Adult; Resequencing microarray; Conjunctivitis; Patients; Human adenovirus; PCR; Culture; Human; Adenoviridae; Conjunctiva disease; Virus; Polymerase chain reaction; Eye disease; Mastadenovirus; Detection
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2009.12.014

Abstract Background Although high-density resequencing microarray is useful for detection and tracking the evolution of viruses associated with respiratory tract infections, no report on using this technology for the detection of viruses in patients with conjunctivitis is available. Objectives To test if high-density resequencing microarray can be applied to detection of viruses in conjunctival swabs for patients with conjunctivitis. Study design In this prospective proof-of-concept study, every 4 or 5 bacterial culture-negative conjunctival swab samples were pooled and subject to viral detection using TessArray™ Resequencing Pathogen Microarrays-Flu 3.1 (RPM-Flu-3.1). Results were compared with human adenovirus (HAdV) hexon gene PCR sequencing and viral culture. Results Thirty-two of the 38 conjunctival swab samples were bacterial culture-negative. Four of the 7 pooled samples were positive for HAdV using RPM-Flu-3.1. Hexon gene PCR sequencing on the 38 original individual samples showed that 3 and 4 samples contained HAdVs species D and B respectively. All the 6 samples that were positive for hexon gene PCR but negative for bacterial culture were also positive by the resequencing microarray. Viral culture was positive for HAdV type 3 in 1 sample, which was also positive by PCR and resequencing microarray. Conclusions Resequencing microarray is as sensitive as PCR for detection of HAdV in conjunctival swabs. Unlike viral culture and hexon gene PCR sequencing, resequencing microarray was not able to differentiate the type and species of HAdV. Development of microarrays for conjunctivitis can be performed for rapid diagnosis of the viral cause of conjunctivitis.