Three-Dimensional Nanostructured Substrates toward Efficient Capture of Circulating Tumor Cells

• Published in: Angewandte Chemie (International ed.) Vol. 48; no. 47; pp. 8970 - 8973
• Main Authors: Wang, Shutao, Wang, Hao, Jiao, Jing, Chen, Kuan-Ju, Owens, Gwen E, Kamei, Ken-ichiro, Sun, Jing, Sherman, David J, Behrenbruch, Christian P, Wu, Hong, Tseng, Hsian-Rong
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01-01-2009; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Antibodies, Immobilized - immunology; Antibodies, Immobilized - metabolism; Antigens, Neoplasm - metabolism; cancer diagnosis; Cell Adhesion Molecules - metabolism; cell capture; Cell Line, Tumor; Epithelial Cell Adhesion Molecule; Fluorescent Dyes - chemistry; Humans; Indoles - chemistry; Microscopy, Electron, Scanning; nanostructures; Nanostructures - chemistry; silicon; Silicon - chemistry
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.200901668

A grabby substrate: A 3D nanostructured substrate, namely, a silicon‐nanopillar (SiNP) array coated with epithelial‐cell adhesion‐molecule antibody (anti‐EpCAM), shows enhanced local topographic interactions between nanoscale cell‐surface components and the substrates surface, resulting in enhanced cell‐capture efficiency when employed to isolate viable cancer cells from whole‐blood samples (see schematic and SEM image of a captured cancer cell).