Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration

• Published in: Journal of tissue engineering Vol. 10; p. 2041731419852703
• Main Authors: Munir, Arooj, Døskeland, Anne, Avery, Steven J, Fuoco, Tiziana, Mohamed-Ahmed, Samih, Lygre, Henning, Finne-Wistrand, Anna, Sloan, Alastair J, Waddington, Rachel J, Mustafa, Kamal, Suliman, Salwa
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 2019; Sage Publications Ltd; SAGE Publishing
• Subjects: Biomedical materials; Bone demineralization; Bone growth; Bone marrow; Bone mass; Bone matrix; Bone morphogenetic protein 2; bone tissue engineering; Collagen; Collagen (type I); Differentiation (biology); drug delivery; functionalisation; Growth factor; Interleukin 6; Interleukin 8; Isotopes; Mass spectrometry; Mass spectroscopy; mesenchymal stem cell; Mineralization; Original; Proteins; Regeneration; Regeneration (physiology); Scaffolds; Stromal cells; Tissue engineering; mesenchymal stem cell; drug delivery; bone tissue engineering; functionalisation; Growth factor
• ISSN: 2041-7314; 2041-7314
• DOI: 10.1177/2041731419852703

Poly(L-lactide-co-ε-caprolactone) scaffolds were functionalised by 10 or 20 µg/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 µg/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.