Genetically Characterized Positive Control Cell Lines Derived from Residual Clinical Blood Samples

• Published in: Clinical chemistry (Baltimore, Md.) Vol. 51; no. 11; pp. 2013 - 2024
• Main Authors: Bernacki, Susan H, Beck, Jeanne C, Stankovic, Ana K, Williams, Laurina O, Amos, Jean, Snow-Bailey, Karen, Farkas, Daniel H, Friez, Michael J, Hantash, Feras M, Matteson, Karla J, Monaghan, Kristin G, Muralidharan, Kasinathan, Pratt, Victoria M, Prior, Thomas W, Richie, Kristy L, Levin, Barbara C, Rohlfs, Elizabeth M, Schaefer, Frederick V, Shrimpton, Antony E, Spector, Elaine B, Stolle, Catherine A, Strom, Charles M, Thibodeau, Stephen N, Cole, Eugene C, Goodman, Barbara K, Stenzel, Timothy T
• Format: Journal Article
• Language: English
• Published: Washington, DC Am Assoc Clin Chem 01-11-2005; American Association for Clinical Chemistry; Oxford University Press
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blood Specimen Collection; Cell Line, Transformed; Cystic fibrosis; Deoxyribonucleic acid; DNA; Epstein-Barr virus; Fundamental and applied biological sciences. Psychology; Genetic Diseases, Inborn - diagnosis; Genetic disorders; Genetic screening; Genetic testing; Genetic Testing - methods; Genetics; Herpesvirus 4, Human; Humans; Investigative techniques, diagnostic techniques (general aspects); Laboratories; Lymphocytes; Lymphocytes - cytology; Medical sciences; Molecular Biology; Mutation; Performance evaluation; Point Mutation; Sequence Deletion; Testing laboratories; Cell line; Clinical biology; Established cell line; Genetics; Biochemistry; Molecular biology; Blood
• ISSN: 0009-9147; 1530-8561
• DOI: 10.1373/clinchem.2005.048694

Positive control materials for clinical diagnostic molecular genetic testing are in critically short supply. High-quality DNA that closely resembles DNA isolated from patient specimens can be obtained from Epstein-Barr virus (EBV)-transformed peripheral blood lymphocyte cell lines. Here we report the development of a process to (a) recover residual blood samples with clinically important mutations detected during routine medical care, (b) select samples likely to provide viable lymphocytes for EBV transformation, (c) establish stable cell lines and confirm the reported mutation(s), and (d) validate the cell lines for use as positive controls in clinical molecular genetic testing applications.
A network of 32 genetic testing laboratories was established to obtain anonymous, residual clinical samples for transformation and to validate resulting cell lines for use as positive controls. Three panel meetings with experts in molecular genetic testing were held to evaluate results and formulate a process that could function in the context of current common practices in molecular diagnostic testing.
Thirteen laboratories submitted a total of 113 residual clinical blood samples with mutations for 14 genetic disorders. Forty-one EBV-transformed cell lines were established. Thirty-five individual point and deletion mutations were shown to be stable after 20 population doublings in culture. Thirty-three cell lines were characterized for specific mutations and validated for use as positive controls in clinical diagnostic applications.
A process for producing and validating positive control cell lines from residual clinical blood samples has been developed. Sustainable implementation of the process could help alleviate the current shortage of positive control materials.