Lobophorin C and D, new kijanimicin derivatives from a marine sponge-associated actinomycetal strain AZS17

• Published in: Marine drugs Vol. 9; no. 3; pp. 359 - 368
• Main Authors: Wei, Rong-Bian, Xi, Tao, Li, Jing, Wang, Ping, Li, Fu-Chao, Lin, Yong-Cheng, Qin, Song
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17-03-2011; Molecular Diversity Preservation International
• Subjects: actinomycete; Aminoglycosides - chemistry; Aminoglycosides - isolation & purification; Aminoglycosides - pharmacology; Animals; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Biological Assay; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Cell Line, Tumor; China; cytotoxic activity; Female; Humans; Liver Neoplasms - drug therapy; Liver Neoplasms - pathology; lobophorin C and D; marine sponge; Oceans and Seas; Porifera - microbiology; Spectrum Analysis; Streptomyces - isolation & purification; Streptomyces - metabolism; China; actinomycete; marine sponge; cytotoxic activity; lobophorin C and D
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md9030359

Marine sponge Hymeniacidon sp. was collected from coastal waters of the East China Sea to isolate symbiotic microorganisms. The resulting sponge-associated actinomycete, Streptomyces carnosus strain AZS17, was cultivated in a 20 L volume of medium for production of bioactive secondary metabolites. Bioassay-guided isolation and purification by varied chromatographic methods yielded two new compounds of kijanimicin derivatives, AS7-2 and AS9-12. Their structures were elucidated by spectroscopy and comparison with literatures. Results showed these two compounds were structurally similar to the previously reported compounds lobophorin A and B, yet differed in specific bond forms, stereochemistry and optical activities. The two novel compounds were named lobophorin C and D. In vitro cytotoxicity investigation by MTT assay indicated their selective activities. Lobophorin C displayed potent cytotoxic activity against the human liver cancer cell line 7402, while lobophorin D showed significant inhibitory effect on human breast cancer cells MDA-MB 435.