Spatially heterogeneous choroid plexus transcriptomes encode positional identity and contribute to regional CSF production

• Published in: The Journal of neuroscience Vol. 35; no. 12; pp. 4903 - 4916
• Main Authors: Lun, Melody P, Johnson, Matthew B, Broadbelt, Kevin G, Watanabe, Momoko, Kang, Young-Jin, Chau, Kevin F, Springel, Mark W, Malesz, Alexandra, Sousa, André M M, Pletikos, Mihovil, Adelita, Tais, Adelita, Tai, Calicchio, Monica L, Zhang, Yong, Holtzman, Michael J, Lidov, Hart G W, Sestan, Nenad, Steen, Hanno, Monuki, Edwin S, Lehtinen, Maria K
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 25-03-2015
• Subjects: Aging - metabolism; Animals; Cerebrospinal Fluid - metabolism; Choroid Plexus - metabolism; Epithelial Cells - metabolism; Female; Fourth Ventricle - metabolism; Humans; Lateral Ventricles - metabolism; Macaca; Macaca mulatta; Male; Mice; Transcriptome; next-generation sequencing; cerebrospinal fluid; choroid plexus
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.3081-14.2015

A sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors into the CSF. To evaluate whether differences in the CSF proteome across ventricles arise, in part, from regional differences in choroid plexus gene expression, we defined the transcriptome of lateral ventricle (telencephalic) versus fourth ventricle (hindbrain) choroid plexus. We find that positional identities of mouse, macaque, and human choroid plexi derive from gene expression domains that parallel their axial tissues of origin. We then show that molecular heterogeneity between telencephalic and hindbrain choroid plexi contributes to region-specific, age-dependent protein secretion in vitro. Transcriptome analysis of FACS-purified choroid plexus epithelial cells also predicts their cell-type-specific secretome. Spatial domains with distinct protein expression profiles were observed within each choroid plexus. We propose that regional differences between choroid plexi contribute to dynamic signaling gradients across the mammalian cerebroventricular system.