Synbiotics suppress the release of lactate dehydrogenase, promote non-specific immunity and integrity of jejunum mucosa in piglets

• Published in: Animal science journal Vol. 87; no. 9; pp. 1157 - 1166
• Main Authors: Andrejčáková, Zuzana, Sopková, Drahomíra, Vlčková, Radoslava, Kulichová, Lucia, Gancarčíková, Soňa, Almášiová, Viera, Holovská, Katarína, Petrilla, Vladimír, Krešáková, Lenka
• Format: Journal Article
• Language: English
• Published: Australia Blackwell Publishing Ltd 01-09-2016; John Wiley and Sons Inc
• Subjects: Additives; Animal diseases; Animal Feed; Animal Nutritional Physiological Phenomena - physiology; Animals; Body temperature; Cell Membrane - ultrastructure; Cell membranes; Cheese; Coronaviridae; Coronavirus; Coronaviruses; Dairy products; Dehydrogenase; Dehydrogenases; E coli; Escherichia coli; Fatty acids; Flax; gut histology; Hematology; Histology; Hogs; Immune status; Immunity; Immunity, Innate; Immunology; Integrity; Intestinal Mucosa - enzymology; Intestinal Mucosa - immunology; Intestinal Mucosa - ultrastructure; Jejunum; Jejunum - enzymology; Jejunum - immunology; Jejunum - ultrastructure; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - metabolism; Lactate dehydrogenase; Lactic acid; Lactobacillus fermentum; Lactobacillus plantarum; Light microscopy; Microscopy, Electron, Transmission; Mucosa; non-specific immunity; Nutrients; Organs; Original; piglet; Polyunsaturated fatty acids; Probiotics; Swine - immunology; Swine - microbiology; synbiotics; Synbiotics - administration & dosage; Weaning; lactate dehydrogenase; piglet; synbiotics; gut histology; non-specific immunity
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12558

The aim of our experiment was to study how synbiotics are able to deal with the problems of post‐weaning piglets. Lactobacillus plantarum – BiocenolTM LP96 (CCM 7512), Lactobacillus fermentum – BiocenolTM LF99 (CCM 7514) and flaxseed (rich in n‐3 polyunsaturated fatty acids) were administered to 36 conventional piglets from a problematic breed with confirmed presence of enterotoxigenic Escherichia coli and Coronavirus. The experimental piglets were supplied with probiotic cheeses and crushed flax‐seed in the period starting 10 days before weaning and lasting up to 14 days post‐weaning. Piglets in the control group were supplied only control cheese. The impact of such additives on the release of lactate dehydrogenase (LDH; spectroscopic and electrophoretic assay), alteration of immunity (index of metabolic activity), jejunum histology (light microscopy), and health of conventional piglets from a problematic breed (monitoring of hematology, consistency and moisture of feces and body temperature) were examined. We found significant decrease in LDH leakage in the blood serum and tissue extracts, indicating better cell membrane integrity in the individual organs of animals. Probiotics and flaxseed applied together seem to be a good source of nutrients to improve the immune status and the integrity of jejunum mucosa during infection. © 2015 Japanese Society of Animal Science