Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection

• Published in: Electrophoresis Vol. 31; no. 16; pp. 2796 - 2803
• Main Authors: Nikcevic, Irena, Piruska, Aigars, Wehmeyer, Kenneth R, Seliskar, Carl J, Limbach, Patrick A, Heineman, William R
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01-08-2010; WILEY-VCH Verlag; WILEY‐VCH Verlag
• Subjects: Benzopyrans; Carboxylic Acids; CCD cameras; Channels; Electrophoresis; Electrophoresis, Capillary - methods; Emission; Fluorescein; Fluorescence detection; Fluorescent Dyes; Hydrogen-Ion Concentration; Kinetics; Lanes; Lasers; Microchip Analytical Procedures; Microfluidics; Monitoring; Multilane microchip; Multiplexing; Naphthols; pKa determinations; Rhodamines; Separation; Spectrometry, Fluorescence - methods
• ISSN: 0173-0835; 1522-2683; 1522-2683
• DOI: 10.1002/elps.201000030

Parallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pKa determination of small molecule analytes is demonstrated with the multilane microchip.