Method of preparing a decellularized porcine tendon using tributyl phosphate

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 96B; no. 2; pp. 199 - 206
• Main Authors: Deeken, C. R., White, A. K., Bachman, S. L., Ramshaw, B. J., Cleveland, D. S., Loy, T. S., Grant, S. A.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-02-2011; Wiley-Blackwell
• Subjects: acellular; Animals; Biological and medical sciences; decellularization; extracellular matrix; Guided Tissue Regeneration - methods; Medical sciences; Methods; Organophosphates - pharmacology; porcine diaphragm tendon; scaffold; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Sus scrofa; Technology. Biomaterials. Equipments; Tendons - drug effects; Tissue Scaffolds; acellular; decellularization; Scaffold; Method; Pig; Vertebrata; Mammalia; Animal; Extracellular matrix; Biomaterial; Artiodactyla; Diaphragm; porcine diaphragm tendon; Ungulata; Biomedical engineering; Tendon
• ISSN: 1552-4973; 1552-4981; 1552-4981
• DOI: 10.1002/jbm.b.31753

Extracellular matrix (ECM) materials are currently utilized for soft tissue repair applications such as vascular grafts, tendon reconstruction, and hernia repair. These materials are derived from tissues such as human dermis and porcine small intestine submucosa, which must be rendered acellular to prevent disease transmission and decrease the risk of an immune response. The ideal decellularization technique removes cells and cellular remnants, but leaves the original collagen architecture intact. The tissue utilized in this study was the central tendon of the porcine diaphragm, which had not been previously investigated for soft tissue repair. Several treatments were investigated during this study including peracetic acid, TritonX‐100, sodium dodecyl sulfate, and tri(n‐butyl) phosphate (TnBP). Of the decellularization treatments investigated, only 1% TnBP was effective in removing cell nuclei while leaving the structure and composition of the tissue intact. Overall, the resulting acellular tissue scaffold retained the ECM composition, strength, resistance to enzymatic degradation, and biocompatibility of the original tissue, making 1% TnBP an acceptable decellularization treatment for porcine diaphragm tendon. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.