Deep learning-based image analysis methods for brightfield-acquired multiplex immunohistochemistry images

• Published in: Diagnostic pathology Vol. 15; no. 1; p. 100
• Main Authors: Fassler, Danielle J, Abousamra, Shahira, Gupta, Rajarsi, Chen, Chao, Zhao, Maozheng, Paredes, David, Batool, Syeda Areeha, Knudsen, Beatrice S, Escobar-Hoyos, Luisa, Shroyer, Kenneth R, Samaras, Dimitris, Kurc, Tahsin, Saltz, Joel
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 28-07-2020; BioMed Central; BMC
• Subjects: Adenocarcinoma; Analysis; Annotations; Antibodies; Antigens; Artificial neural networks; Automation; Beta cells; Biomarkers; Biomarkers, Tumor - analysis; Cancer; Carcinoma, Pancreatic Ductal - immunology; Carcinoma, Pancreatic Ductal - pathology; CD16 antigen; CD20 antigen; CD3 antigen; CD4 antigen; CD8 antigen; Color; Color sensitivity; Deconvolution; Deep Learning; Digital imaging; Digital pathology image analysis; Formaldehyde; Ground truth; Humans; Image acquisition; Image analysis; Image processing; Image Processing, Computer-Assisted - methods; Image segmentation; Immune system; Immunohistochemistry; Immunohistochemistry - methods; Instrumentation; Lymphocytes T; Machine learning; Macrophages; Methods; Multiplex immunohistochemistry; Multiplexing; Neural networks; Pancreas; Pancreatic Neoplasms - immunology; Pancreatic Neoplasms - pathology; Paraffin; Paraffins; Pathology; Populations; Predictions; Spatial analysis; Spatial distribution; Tumor cells; Tumor immune microenvironment; Tumors; Deep learning; Tumor immune microenvironment; Digital pathology image analysis; Multiplex immunohistochemistry
• ISSN: 1746-1596; 1746-1596
• DOI: 10.1186/s13000-020-01003-0

Multiplex immunohistochemistry (mIHC) permits the labeling of six or more distinct cell types within a single histologic tissue section. The classification of each cell type requires detection of the unique colored chromogens localized to cells expressing biomarkers of interest. The most comprehensive and reproducible method to evaluate such slides is to employ digital pathology and image analysis pipelines to whole-slide images (WSIs). Our suite of deep learning tools quantitatively evaluates the expression of six biomarkers in mIHC WSIs. These methods address the current lack of readily available methods to evaluate more than four biomarkers and circumvent the need for specialized instrumentation to spectrally separate different colors. The use case application for our methods is a study that investigates tumor immune interactions in pancreatic ductal adenocarcinoma (PDAC) with a customized mIHC panel.
Six different colored chromogens were utilized to label T-cells (CD3, CD4, CD8), B-cells (CD20), macrophages (CD16), and tumor cells (K17) in formalin-fixed paraffin-embedded (FFPE) PDAC tissue sections. We leveraged pathologist annotations to develop complementary deep learning-based methods: (1) ColorAE is a deep autoencoder which segments stained objects based on color; (2) U-Net is a convolutional neural network (CNN) trained to segment cells based on color, texture and shape; and ensemble methods that employ both ColorAE and U-Net, collectively referred to as (3) ColorAE:U-Net. We assessed the performance of our methods using: structural similarity and DICE score to evaluate segmentation results of ColorAE against traditional color deconvolution; F1 score, sensitivity, positive predictive value, and DICE score to evaluate the predictions from ColorAE, U-Net, and ColorAE:U-Net ensemble methods against pathologist-generated ground truth. We then used prediction results for spatial analysis (nearest neighbor).
We observed that (1) the performance of ColorAE is comparable to traditional color deconvolution for single-stain IHC images (note: traditional color deconvolution cannot be used for mIHC); (2) ColorAE and U-Net are complementary methods that detect 6 different classes of cells with comparable performance; (3) combinations of ColorAE and U-Net into ensemble methods outperform using either ColorAE and U-Net alone; and (4) ColorAE:U-Net ensemble methods can be employed for detailed analysis of the tumor microenvironment (TME). We developed a suite of scalable deep learning methods to analyze 6 distinctly labeled cell populations in mIHC WSIs. We evaluated our methods and found that they reliably detected and classified cells in the PDAC tumor microenvironment. We also present a use case, wherein we apply the ColorAE:U-Net ensemble method across 3 mIHC WSIs and use the predictions to quantify all stained cell populations and perform nearest neighbor spatial analysis. Thus, we provide proof of concept that these methods can be employed to quantitatively describe the spatial distribution immune cells within the tumor microenvironment. These complementary deep learning methods are readily deployable for use in clinical research studies.