Peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine

• Published in: BMC veterinary research Vol. 17; no. 1; p. 51
• Main Authors: Wu, Qi, Lin, Zhixian, Wu, Jinsen, Qian, Kun, Shao, Hongxia, Ye, Jianqiang, Qin, Aijian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 25-01-2021; BioMed Central; BMC
• Subjects: Animals; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Antigens; Bronchitis; Chickens - immunology; Chickens - virology; Coronavirus infections; Coronavirus Infections - immunology; Coronavirus Infections - prevention & control; Coronaviruses; Cross-protection; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzymes; Evaluation; Genomes; Glycoproteins; Health aspects; Identification and classification; Immune response; Infections; Infectious bronchitis virus; Infectious bronchitis virus - immunology; Methods; Neutralization Tests - methods; Neutralizing antibody; Peptide ELISA; Peptides; Physiological aspects; Poultry; Poultry Diseases - immunology; Poultry Diseases - prevention & control; Poultry Diseases - virology; Prevention; Proteins; Reproducibility of Results; Respiratory diseases; Sensitivity and Specificity; Serology; Serotypes; Severe acute respiratory syndrome coronavirus 2; Vaccination; Vaccines; Viral antibodies; Viral vaccines; Virulence; Viruses; China; Evaluation; Peptide ELISA; Neutralizing antibody; Infectious bronchitis virus
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-021-02757-5

Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies.
In this study, a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine was developed. The pELISA could indirect evaluate neutralizing antibody titers against different types of IBV in all tested sera. The titers measured with the pELISA had a coefficient of 0.83 for neutralizing antibody titers.
The pELISA could detect antibodies against different types of IBV in all tested sera. The pELISA has the potential to evaluate samples for IBV-specific neutralizing antibodies and surveillance the infection of IBV.