Identification of human renal cell carcinoma associated genes by suppression subtractive hybridization

• Published in: British journal of cancer Vol. 85; no. 9; pp. 1372 - 1382
• Main Authors: STASSAR, M. J. J. G, DEVITT, G, BROSIUS, M, RINNAB, L, PRANG, J, SCHRADIN, T, SIMON, J, PETERSEN, S, KOPP-SCHNEIDER, A, ZÖLLER, M
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 02-11-2001
• Subjects: Biological and medical sciences; Biomarkers, Tumor - analysis; Blotting, Northern; Cancer research; Carcinoma, Renal Cell - genetics; Cell Adhesion; Cell Survival; DNA Primers; DNA, Neoplasm - genetics; Gene expression; Gene Expression Regulation, Neoplastic; Growth factors; Humans; Hybridization; Kidney cancer; Kidney Neoplasms - genetics; Kidneys; Medical prognosis; Medical research; Medical sciences; Metastasis; Neovascularization, Pathologic; Nephrology. Urinary tract diseases; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; Proteins; Regular; Research centers; Tumor Cells, Cultured; Tumors of the urinary system; Germany; Kidney disease; Human; Urinary system disease; Exploration; Malignant tumor; Gene expression; Kidney; Tissue; Chemotherapy; Target; Treatment; Gene; Established cell line; Genetics; Grawitz tumor
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1054/bjoc.2001.2074

Renal cell carcinoma (RCC) are frequently chemo- and radiation resistant. Thus, there is a need for identifying biological features of these cells that could serve as alternative therapeutic targets. We performed suppression subtractive hybridization (SSH) on patient-matched normal renal and RCC tissue to identify variably regulated genes. 11 genes were strongly up-regulated or selectively expressed in more than one RCC tissue or cell line. Screening of filters containing cancer-related cDNAs confirmed overexpression of 3 of these genes and 3 additional genes were identified. These 14 differentially expressed genes, only 6 of which have previously been associated with RCC, are related to tumour growth/survival (EGFR, cyclin D1, insulin-like growth factor-binding protein-1 and a MLRQ sub-unit homologue of the NADH:ubiquinone oxidoreductase complex), angiogenesis (vascular endothelial growth factor, endothelial PAS domain protein-1, ceruloplasmin, angiopoietin-related protein 2) and cell adhesion/motility (protocadherin 2, cadherin 6, autotaxin, vimentin, lysyl oxidase and semaphorin G). Since some of these genes were overexpressed in 80-90% of RCC tissues, it is important to evaluate their suitability as therapeutic targets.