Ketamine induces apoptosis via the mitochondrial pathway in human lymphocytes and neuronal cells

• Published in: British journal of anaesthesia : BJA Vol. 105; no. 3; pp. 347 - 354
• Main Authors: Braun, S, Gaza, N, Werdehausen, R, Hermanns, H, Bauer, I, Durieux, M.E., Hollmann, M.W., Stevens, M.F.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01-09-2010; Oxford University Press
• Subjects: anaesthetics i.v. ketamine; Anesthesia; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; anesthetic i.v. stereoisomers; Anesthetics, Dissociative - pharmacology; Annexin V; Apoptosis - drug effects; Apoptosis - physiology; Biological and medical sciences; Dose-Response Relationship, Drug; flowmetry; Humans; Jurkat Cells; Ketamine - pharmacology; measurement techniques; Medical sciences; Mitochondria - drug effects; Mitochondria - physiology; Necrosis; Neurons - drug effects; Neurons - pathology; neurotoxicity; Signal Transduction - drug effects; Signal Transduction - physiology; T-Lymphocytes - drug effects; T-Lymphocytes - pathology; toxicity; Tumor Cells, Cultured; measurement techniques; toxicity; anaesthetics i.v. ketamine; flowmetry; neurotoxicity; anesthetic i.v. stereoisomers; Human; anesthetic i.v stereoisomers; Toxicity; Glutamate receptor; Ketamine; General anesthetic; Measurement technique; Anesthesia; anaesthetics i.v ketamine; Antagonist; NMDA receptor; Flowmetry; Lymphocyte; Apoptosis
• ISSN: 0007-0912; 1471-6771
• DOI: 10.1093/bja/aeq169

Ketamine has been shown to have neurotoxic properties, when administered neuraxially. The mechanism of this local toxicity is still unknown. Therefore, we investigated the mechanism of cytotoxicity in different human cell lines in vitro.
We incubated the following cell types for 24 h with increasing concentrations of S(+)-ketamine and racemic ketamine: (i) human Jurkat T-lymphoma cells overexpressing the antiapoptotic B-cell lymphoma 2 protein, (ii) cells deficient of caspase-9, caspase-8, or Fas-associated protein with death domain and parental cells, and (iii) neuroblastoma cells (SHEP). N-Methyl-d-aspartate (NMDA) receptors and caspase-3 cleavage were identified by immunoblotting. Cell viability and apoptotic cell death were evaluated flowcytometrically by Annexin V and 7-aminoactinomycin D double staining. Mitochondrial metabolic activity and caspase-3 activation were measured.
Ketamine, in a concentration-dependent manner, induced apoptosis in lymphocytes and neuroblastoma cell lines. Cell lines with alterations of the mitochondrial pathway of apoptosis were protected against ketamine-induced apoptosis, whereas alterations of the death receptor pathway did not reduce apoptosis. S(+)-Ketamine and racemic ketamine induced the same percentage of cell death in Jurkat cells, whereas in neuroblastoma cells, S(+)-ketamine was slightly less toxic.
Ketamine at millimolar concentrations induces apoptosis via the mitochondrial pathway, independent of death receptor signalling. At higher concentrations necrosis is the predominant mechanism. Less toxicity of S(+)-ketamine was observed in neuroblastoma cells, but this difference was minor and therefore unlikely to be mediated via the NMDA receptor.