Microbial-derived imidazole propionate links the heart failure-associated microbiome alterations to disease severity

• Published in: Genome medicine Vol. 16; no. 1; pp. 27 - 12
• Main Authors: Raju, Sajan C, Molinaro, Antonio, Awoyemi, Ayodeji, Jørgensen, Silje F, Braadland, Peder R, Nendl, Andraz, Seljeflot, Ingebjørg, Ueland, Per M, McCann, Adrian, Aukrust, Pål, Vestad, Beate, Mayerhofer, Cristiane, Broch, Kaspar, Gullestad, Lars, Lappegård, Knut T, Halvorsen, Bente, Kristiansen, Karsten, Hov, Johannes R, Trøseid, Marius
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 08-02-2024; BioMed Central; Springer Nature; BMC
• Subjects: Cardiovascular diseases; Comparative analysis; Congestive heart failure; Development and progression; Digestive system; Disease; Dysbacteriosis; Dysbiosis; Ethylenediaminetetraacetic acid; Etiology; Fatty acids; Food; Gastrointestinal tract; Genomes; Gut microbiota; Heart failure; Heart Failure - metabolism; Histidine; Humans; Imidazole; Imidazole propionate; Imidazoles; Inflammation; Intestinal microflora; Metabolism; Metabolites; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Nutrition research; Patient Acuity; Permeability; Physiological aspects; Propionic acid; Proteins; Questionnaires; Taxonomy; Type 2 diabetes; Germany; Gut microbiota; Heart failure; Inflammation; Imidazole propionate
• ISSN: 1756-994X; 1756-994X
• DOI: 10.1186/s13073-024-01296-6

Interactions between the gut microbiota, diet, and host metabolism contribute to the development of cardiovascular disease, but a firm link between disease-specific gut microbiota alterations and circulating metabolites is lacking.
We performed shot-gun sequencing on 235 samples from 166 HF patients and 69 healthy control samples. Separate plasma samples from healthy controls (n = 53) were used for the comparison of imidazole propionate (ImP) levels. Taxonomy and functional pathways for shotgun sequencing data was assigned using MetaPhlAn3 and HUMAnN3 pipelines.
Here, we show that heart failure (HF) is associated with a specific compositional and functional shift of the gut microbiota that is linked to circulating levels of the microbial histidine-derived metabolite ImP. Circulating ImP levels are elevated in chronic HF patients compared to controls and associated with HF-related gut microbiota alterations. Contrary to the microbiota composition, ImP levels provide insight into etiology and severity of HF and also associate with markers of intestinal permeability and systemic inflammation.
Our findings establish a connection between changes in the gut microbiota, the presence, etiology, and severity of HF, and the gut-microbially produced metabolite ImP. While ImP appears promising as a circulating biomarker reflecting gut dysbiosis related to HF, further studies are essential to demonstrate its causal or contributing role in HF pathogenesis.
NCT02637167, registered December 22, 2015.