Genetic and Functional Differences between Multipotent Neural and Pluripotent Embryonic Stem Cells

Stem cells (SCs) are functionally defined by their abilities to self-renew and generate differentiated cells. Although much effort has been focused on defining the common characteristics among various types of SCs, the genetic and functional differences between multipotent and pluripotent SCs have g...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 100; no. Suppl 1; pp. 11866 - 11872
Main Authors:	D'Amour, Kevin A., Gage, Fred H.
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 30-09-2003 National Acad Sciences
Series:	Colloquium Paper
Subjects:	Animals Base Sequence Blastocyst Cell cycle Colloquium Papers Cultured cells DNA, Complementary - genetics DNA-Binding Proteins - genetics Embryonic stem cells Embryos Fetus Fluorescence Gene Expression Profiling Genes Genetics Green Fluorescent Proteins HMGB Proteins Luminescent Proteins - genetics Mice Mice, Transgenic Multipotent stem cells Multipotent Stem Cells - cytology Multipotent Stem Cells - physiology Neurons Neurons - chemistry Neurons - physiology Nuclear Proteins - genetics Pluripotent stem cells Pluripotent Stem Cells - cytology Pluripotent Stem Cells - physiology Promoter Regions, Genetic Recombinant Fusion Proteins - genetics Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Stem cells Transcription Factors
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Summary:	Stem cells (SCs) are functionally defined by their abilities to self-renew and generate differentiated cells. Although much effort has been focused on defining the common characteristics among various types of SCs, the genetic and functional differences between multipotent and pluripotent SCs have garnered less attention. We report a direct genetic and functional comparison of molecularly defined and clonally related populations of neural SCs (NSCs) and embryonic SCs (ESCs), using the Sox2 promoter for isolation of purified populations by fluorescence-activated cell sorting. A stringent expression profile comparison of promoter-defined NSCs and ESCs revealed a striking dissimilarity, and subsequent chimera analyses confirmed the fundamental differences in cellular potency between these populations. This direct comparison elucidates the molecular basis for the functional differences in pluripotent ESCs and multipotent NSCs.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 This paper results from the Arthur M. Sackler Colloquium of the National Academy of Sciences, “Regenerative Medicine,” held October 18-22, 2002, at the Arnold and Mabel Beckman Center of the National Academies of Science and Engineering in Irvine, CA. Abbreviations: SC, stem cell; EGFP, enhanced GFP; En, embryonic day n; ESC, embryonic SC; FACS, fluorescence-activated cell sorting; ICM, inner cell mass; NSC, neural stem cell; NSCc, cultured NSC; NSCf, freshly isolated NSC. To whom correspondence should be addressed. E-mail: gage@salk.edu.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1834200100