Embryos derived from donor or patient oocytes are not different for in vitro fertilization outcomes when PGT allows euploid embryo selection: a retrospective study

• Published in: Clinical and translational medicine Vol. 9; no. 1; pp. 1 - 10
• Main Authors: Schaeffer, Elizabeth, Porchia, Leonardo M., Neumann, Adina, Luna, Almena, Rojas, Tania, López-Bayghen, Esther
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 07-02-2020; John Wiley & Sons, Inc; Wiley
• Subjects: Age; Aneuploidy; Biopsy; Clinical medicine; Clinical outcomes; Embryos; Genomes; Implantation; In vitro fertilization; Medical diagnosis; Medicine; Medicine & Public Health; Patients; Pregnancy; Preimplantation genetic diagnosis; Ultrasonic imaging; Aneuploidy; Implantation; Preimplantation genetic diagnosis
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1186/s40169-020-0266-1

Background
At our facilities, patients that received embryos using donor oocyte during in vitro fertilization (IVF), usually have had at least one failed attempt to produce at least one euploid embryo with their own oocytes; however, the current debate between using donor over patient oocytes remains inconclusive. We examined the aneuploidy rate and IVF clinical outcomes from embryos derived from either donor or patient oocytes.
Methods
Retrospectively, 973 cycles were examined of patients who underwent a standard IVF protocol. Chromosomal content was determined using Pre-implantation Genetic Testing (PGT) by either microarray-comparative genomic hybridization or Next-generation sequencing from either Day 3 (blastocysts) or Day 5 (trophectoderm) embryo biopsies, respectively. Embryo implantation was confirmed by serum β-hCG (> 10 m IU/mL/Day 14), whereas clinical pregnancy by a fetal heartbeat (Week 6.5–8).
Results
Embryos derived from donor oocytes presented with more monosomies than embryos derived from patient oocytes (41.2% vs. 25.4%, p < 0.05, respectively); however, only Trisomy 7 (0.4% vs. 2.3%, p < 0.05) and Trisomy in X (0.7% vs. 2.3%, p < 0.05) were significantly less present when compared to patient oocyte derived embryos. Interestingly, rates for embryo implantation (46.7% vs. 50.8%, p = 0.35), clinical pregnancy (38.5% vs. 43.1%, p = 0.30), and live birth (30.5% vs. 30.5%, p = 0.99) were similar for embryos derived from donor and patient oocytes. These results did not change when adjusted for the number of embryos implanted.
Conclusion
Here, we show no significant differences in achieving pregnancy when using donor oocytes. Taking into consideration that aneuploidy rates are > 30% in embryos, independent of the oocyte origin, PGT should be recommended with donor oocytes as well.