Population dynamics and antigenic drift of Bordetella pertussis following whole cell vaccine replacement, Barcelona, Spain, 1986-2015

• Published in: Emerging microbes & infections Vol. 8; no. 1; pp. 1711 - 1720
• Main Authors: Mir-Cros, Alba, Moreno-Mingorance, Albert, Martín-Gómez, M. Teresa, Codina, Gema, Cornejo-Sánchez, Thais, Rajadell, Mireia, Van Esso, Diego, Rodrigo, Carlos, Campins, Magda, Jané, Mireia, Pumarola, Tomàs, Fàbrega, Anna, González-López, Juan José
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01-01-2019; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: antigenic variants; Antigenic Variation; Bacterial Outer Membrane Proteins - administration & dosage; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - genetics; Bacterial Vaccines - immunology; Bordetella pertussis - genetics; Bordetella pertussis - immunology; Genotype; Humans; Minisatellite Repeats; MLVA; Original; pertussis vaccine; PFGE; Population; Population Dynamics; Spain; Vaccines; Virulence Factors, Bordetella - administration & dosage; Virulence Factors, Bordetella - genetics; Virulence Factors, Bordetella - immunology; Whooping cough; Whooping Cough - microbiology; Whooping Cough - prevention & control; Spain; PFGE; antigenic variants; pertussis vaccine; MLVA; Whooping cough
• ISSN: 2222-1751; 2222-1751
• DOI: 10.1080/22221751.2019.1694395

Among the factors associated with the resurgence of whooping cough, special emphasis has been given to pathogen adaptation after the introduction of the acellular vaccine (ACV). To assess the impact of the vaccine transition strategy from whole-cell vaccine (WCV) to ACV on population dynamics of Bordetella pertussis in Barcelona (Spain), we studied 339 isolates collected from 1986 to 2015 by PFGE and multi-locus variable-number tandem repeat analysis (MLVA). Additionally, allelic variants for the pertussis toxin and its promoter, pertactin, type 3 fimbriae and fimbrial serotyping were assessed to determine its antigenic drift. A shift was observed in the B. pertussis population as well as in its antigenic profile concurrently with the introduction of ACV in Barcelona. Four out of the five most prevalent PFGE profiles were replaced by new profiles following the ACV introduction. MLVA type 27 was the dominant genotype, and its frequency increased from 25% to 79.3% after WCV replacement. Antigen typing demonstrated the emergence of prn2, ptxP3, fim3-2 and a shift from the fimbriae 3 to the fimbriae 2 serotypes after the ACV introduction. Our findings support the presence of population and antigenic dynamic changes in B. pertussis likely driven by the introduction of ACV.