Complete chloroplast genome sequence of Pueraria lobata (Willd.) Ohwi (Fabaceae): a traditional Chinese medicinal herb

• Published in: Mitochondrial DNA. Part B. Resources Vol. 5; no. 1; pp. 25 - 26
• Main Authors: Sun, Jiahui, Wang, Ling, Yuan, Yuan, Zhou, Rongrong, Duan, Haiyan, Zhao, Yuping
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-01-2020; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Chloroplast genome; Chloroplasts; Genomes; genomic resources; Herbal medicine; Medicinal plants; Mitogenome Announcement; Next-generation sequencing; Nucleotide sequence; Pueraria lobata; rRNA; tRNA; genomic resources; Pueraria lobata; Chloroplast genome
• ISSN: 2380-2359; 2380-2359
• DOI: 10.1080/23802359.2019.1694850

Pueraria lobata is a traditional Chinese herb which has been used medically since ancient times. In this study, we sequenced the complete chloroplast genome of P. lobata based on next-generation sequencing and used the data to assess genomic resources. The chloroplast genome of P. lobata is 153,411 bp in length consisting of large and small single-copy regions of length 84,142 and 17,989 bp, separated by two IR regions of 25,640 bp. The overall GC content was 35.4%. De novo assembly and annotation showed the presence of 112 unique genes with 78 protein-coding genes, 30 tRNA genes, and four rRNA genes. A maximum-likelihood phylogenomic analysis showed that Pueraria was sister to Pediomelum + Glycine.