Improving the Cellulose Enzymatic Digestibility of Sugarcane Bagasse by Atmospheric Acetic Acid Pretreatment and Peracetic Acid Post-Treatment

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 12; p. 4689
• Main Authors: Bai, Yuchen, Tian, Mingke, Dai, Zhiwei, Zhao, Xuebing
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01-06-2023; MDPI
• Subjects: Acetic Acid; acetic acid delignification; Acetylation; Analysis; Bagasse; Biomass; Carbohydrates; Catalysts; Cellulase; Cellulose; Digestibility; Enzymes; Hydrolysis; kinetic modeling; Kinetics; Lignin; Lignocellulose; lignocellulosic biomass; Organic acids; Oxidation; Peracetic acid; Peracetic Acid - pharmacology; peracetic acid post-treatment; Polysaccharides; Pretreatment; Response surface methodology; Saccharum; Statistical analysis; Sugarcane; sugarcane bagasse; Sulfuric acid; Temperature; Temperature effects; China; lignocellulosic biomass; sugarcane bagasse; peracetic acid post-treatment; acetic acid delignification; pretreatment; kinetic modeling
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28124689

Pretreatment of sugarcane bagasse (SCB) by aqueous acetic acid (AA), with the addition of sulfuric acid (SA) as a catalyst under mild condition (<110 °C), was investigated. A response surface methodology (central composite design) was employed to study the effects of temperature, AA concentration, time, and SA concentration, as well as their interactive effects, on several response variables. Kinetic modeling was further investigated for AA pretreatment using both Saeman's model and the Potential Degree of Reaction (PDR) model. It was found that Saeman's model showed a great deviation from the experimental results, while the PDR model fitted the experimental data very well, with determination coefficients of 0.95-0.99. However, poor enzymatic digestibility of the AA-pretreated substrates was observed, mainly due to the relatively low degree of delignification and acetylation of cellulose. Post-treatment of the pretreated cellulosic solid well improved the cellulose digestibly by further selectively removing 50-60% of the residual linin and acetyl group. The enzymatic polysaccharide conversion increased from <30% for AA-pretreatment to about 70% for PAA post-treatment.