A Novel Strategy for Rapid Fluorescence Detection of FluB and SARS-CoV-2

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 5; p. 2104
• Main Authors: Yang, Zhijin, Xue, Zhiwei, Zheng, Kejie, Zhang, Yule, Sui, Guorong, Yang, Haima, Zhuang, Songlin, Zheng, Lulu, Zhang, Dawei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23-02-2023; MDPI
• Subjects: Antibodies; Antigens; Binding sites; China; COVID-19; Epidemics; FluB; Fluorescence; Health aspects; Humans; immunochromatographic test strip; Immunofluorescence; Infections; Influenza; Limit of Detection; Microspheres; Outbreaks; Portable equipment; Proteins; Quantum dots; Quantum Dots - chemistry; rapid fluorescence detection; Resource allocation; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Smartphones; Ultraviolet radiation; Virus diseases; Viruses; China; FluB; SARS-CoV-2; immunochromatographic test strip; rapid fluorescence detection
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28052104

Undoubtedly, SARS-CoV-2 has caused an outbreak of pneumonia that evolved into a worldwide pandemic. The confusion of early symptoms of the SARS-CoV-2 infection with other respiratory virus infections made it very difficult to block its spread, leading to the expansion of the outbreak and an unreasonable demand for medical resource allocation. The traditional immunochromatographic test strip (ICTS) can detect one analyte with one sample. Herein, this study presents a novel strategy for the simultaneous rapid detection of FluB/SARS-CoV-2, including quantum dot fluorescent microspheres (QDFM) ICTS and a supporting device. The ICTS could be applied to realize simultaneous detection of FluB and SARS-CoV-2 with one test in a short time. A device supporting FluB/SARS-CoV-2 QDFM ICTS was designed and had the characteristics of being safe, portable, low-cost, relatively stable, and easy to use, ensuring the device could replace the immunofluorescence analyzer in cases where there is no need for quantification. This device does not need to be operated by professional and technical personnel and has commercial application potential.