Morphology, Cytotoxicity, and Antimicrobial Activity of Electrospun Polycaprolactone Biomembranes with Gentamicin and Nano-Hydroxyapatite

• Published in: Membranes (Basel) Vol. 14; no. 1; p. 10
• Main Authors: Mirica, Ioana-Codruta, Furtos, Gabriel, Moldovan, Marioara, Prodan, Doina, Petean, Ioan, Campian, Radu-Septimiu, Pall, Emoke, Lucaciu, Ondine
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-12-2023; MDPI
• Subjects: Aminoglycosides; Antibacterial agents; Antimicrobial activity; Bone growth; Bones; Cytotoxicity; Dental implants; E coli; Electrospinning; Fibers; Gentamicin; guided bone regeneration; Hydroxyapatite; Molecular weight; Morphology; nano-hydroxyapatite; Nanocomposites; Penicillin; Polycaprolactone; Polymers; Regeneration; Regeneration (physiology); SEM; Toxicity; Romania; United States > US; Japan; gentamicin; guided bone regeneration; nano-hydroxyapatite; SEM; electrospinning; polycaprolactone
• ISSN: 2077-0375; 2077-0375
• DOI: 10.3390/membranes14010010

The aim of this research is to develop new nanocomposite membranes (NMs) for guided bone regeneration from polycaprolactone (PCL), with different concentrations of gentamicin sulfate (GEN) and nano-hydroxyapatite (nHAP) through electrospinning. The obtained NMs were characterized for structure through SEM and AFM, which revealed the influence of GEN and nHAP on the fiber diameter. The addition of GEN lowered the fiber diameter, and the addition of nHAP increased the diameter of the fibers. The NMs demonstrated antibacterial properties against
,
,
, and
depending on the drug concentration, while being negligibly affected by the nHAP content. NM cytotoxicity assessment, performed once using the MTT assay, revealed no cytotoxicity. The developed NMs could be a promising alternative for guided bone regeneration.