Molecular diversity of the faecal microbiota of Toy Poodles in Japan

• Published in: Journal of Veterinary Medical Science Vol. 80; no. 5; pp. 749 - 754
• Main Authors: OMATSU, Tsutomu, OMURA, Miki, KATAYAMA, Yukie, KIMURA, Toru, OKUMURA, Maho, OKUMURA, Atsushi, MURATA, Yoshiteru, MIZUTANI, Tetsuya
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-05-2018; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: 16S rRNA gene barcoding; DNA barcoding; faecal microbiota; Genetics; Internal Medicine; Intestinal microflora; Intestine; Microbiota; Molecular chains; Next-generation sequencing; rRNA 16S; Toy Poodle; 16S rRNA gene barcoding; toy poodle; faecal microbiota
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.17-0582

The intestinal microbiota was revealed with the recent advances in molecular techniques, such as high-throughput sequencing analysis. As a result, the microbial changes are thought to influence the health of humans and animals and such changes are affected by several factors including diet, genetics, age, sex, and diseases. Similar studies are being conducted in dogs, and the knowledge of intestinal microbiota in dogs is expanding. Nonetheless, basic information on intestinal microbiota in dogs is less than that of humans. Our aim was to study toy poodles (n=21), a popular companion dog, in terms of basic characteristics of the faecal microbiota by 16S rRNA gene barcoding analysis. In the faecal microbiota, Firmicutes, Bacteroidetes, Proteobacteria, and Fusobacteria were the dominant phyla (over 93.4% of faecal microbiota) regardless of the attributes of the dogs. In family level, Enterobacteriaceae, Bacteroidaceae, and Lachnospiraceae were most prevalent. In case of a dog with protein-losing enteropathy, the diversity of faecal microbiota was different between before and after treatment. This study provides basic information for studying on faecal microbiota in toy poodles.