Development of Two-Channel Phosphorescent Core-Shell Nanoprobe for Ratiometric and Time-Resolved Luminescence Imaging of Intracellular Oxygen Levels

• Published in: Particle & particle systems characterization Vol. 32; no. 1; pp. 48 - 53
• Main Authors: Sun, Huibin, Zhang, Jie, Zhang, Kenneth Yin, Liu, Shujuan, Liang, Hua, Lv, Wen, Qiao, Weili, Liu, Xiangmei, Yang, Tianshe, Zhao, Qiang, Huang, Wei
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01-01-2015; Wiley Subscription Services, Inc
• Subjects: Biocompatibility; Imaging; imaging agents; Luminescence; Monitoring; nanoparticles; Nanostructure; Oxygen; Phosphorescence; sensors; Silicon dioxide; Strategy
• ISSN: 0934-0866; 1521-4117
• DOI: 10.1002/ppsc.201400106

An effective strategy is developed to realize a ratiometric oxygen nanoprobe with two‐channel phosphorescence signals based on a core–shell silica nanoparticle. This nanoprobe exhibits good water dispersibility and biocompatibility. Bioimaging experiments demonstrate that the nanoprobe can be used for monitoring the change in intracellular O2 concentration and performing quantitative O2 measurement through ratiometric and time‐resolved luminescence bioimaging.