Evaluation of the Panbio dengue virus nonstructural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos

• Published in: Diagnostic microbiology and infectious disease Vol. 60; no. 1; pp. 43 - 49
• Main Authors: Blacksell, Stuart D, Mammen, Mammen P, Thongpaseuth, Soulignasack, Gibbons, Robert V, Jarman, Richard G, Jenjaroen, Kemajittra, Nisalak, Ananda, Phetsouvanh, Rattanaphone, Newton, Paul N, Day, Nicholas P.J
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 2008; Elsevier
• Subjects: Antibodies, Viral - blood; Antigens, Viral - blood; Arboviroses; Biological and medical sciences; Dengue; Dengue - diagnosis; Dengue fevers; Dengue virus; Dengue Virus - immunology; Dengue Virus - isolation & purification; Diagnosis; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Human viral diseases; Humans; IgM; Immunochromatographic; Immunoglobulin M - blood; Infectious Disease; Infectious diseases; Internal Medicine; Laos; Medical sciences; Microbiology; Miscellaneous; NS1; Point-of-care; Sensitivity and Specificity; Serology; Tropical viral diseases; Viral diseases; Viral Nonstructural Proteins - blood; Virology; Asia; Laos; Dengue; Immunochromatographic; Serology; Diagnosis; Point-of-care; Laos; NS1; IgM; Antibody; Acute; Dengue virus 1; Flavivirus; Infection; Virus; Antigen; Viral disease; Dengue group virus; Arbovirus disease; Flaviviridae; Detection; ELISA assay
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2007.07.011

Abstract We evaluated 2 commercial enzyme-linked immunosorbent assays (ELISAs) for the diagnosis of dengue infection; one a serologic test for immunoglobulin M (IgM) antibodies, the other based on detection of dengue virus nonstructural 1 (NS1) antigen. Using gold standard reference serology on paired sera, 41% (38/92 patients) were dengue confirmed, with 4 (11%) acute primary and 33 (87%) acute secondary infections (1 was of indeterminate status). Sensitivity of the NS1-ELISA was 63% (95% confidence interval [CI], 53–73) on admission samples but was much less sensitive (5%; 95% CI, 1–10) on convalescent samples. The IgM capture ELISA had a lower but statistically equivalent sensitivity compared with the NS1-ELISA for admission samples (45%; 95% CI, 35–55) but was more sensitive on convalescent samples (58%; 95% CI, 48–68). The results of the NS1 and IgM capture ELISAs were combined using a logical OR operator, increasing the sensitivity for admission samples (79%; 95% CI, 71–87), convalescent samples (63%; 95% CI, 53–73), and all samples (71%; 95% CI, 65–78).