The est1 Regulation Depends on the Oxygen Concentration in Acetobacter pasteurianus

• Published in: Bioscience, biotechnology, and biochemistry Vol. 65; no. 3; pp. 725 - 727
• Main Authors: KASHIMA, Yasuhiro, NAKAJIMA, Yusuke, KOSUGI, Akihiko, TAYAMA, Kenji, KOIZUMI, Yukimichi, UDAKA, Shigezo, YANAGIDA, Fujiharu
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-03-2001; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Acetobacter pasteurianus; Alcohol Dehydrogenase - genetics; Bacteriology; Base Sequence; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; est1 gene; esterase; Esterases - genetics; Ethanol; FNR; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Genetics; Microbiology; Mission oriented research; Molecular Sequence Data; Oxygen - metabolism; transcriptional regulation; Acetobacteraceae; Oxygen; Regulation(control); Ethanol; Transcription; Enzyme; Acetobacter pasteurianus; Concentration effect; Bacteria; Hydrolases; Esterases; Gene expression
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.65.725

The regulation mechanism for expression of the ethanol inducible esterase gene, est1, was investigated in A. pasteurianus. Deletion analysis of the 5' non coding region of est1 showed that the FNR-binding consensus sequence is important in the induction of est1 by ethanol. Cells grown under oxygen starvation produced esterase-1 in not only the presence but also the absence of ethanol. These results suggest that the induction of est1-expression depends on the oxygen concentration, and the gene may be induced by a FNR-like factor activated by a decrease in the intracellular oxygen concentration.