FSH and Its Second Messenger cAMP Stimulate the Transcription of Human Anti-Müllerian Hormone in Cultured Granulosa Cells

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 25; no. 4; pp. 645 - 655
• Main Authors: Taieb, Joëlle, Grynberg, Michaël, Pierre, Alice, Arouche, Nassim, Massart, Perrine, Belville, Corinne, Hesters, Laetitia, Frydman, René, Catteau-Jonard, Sophie, Fanchin, Renato, Picard, Jean-Yves, Josso, Nathalie, Rey, Rodolfo A, di Clemente, Nathalie
• Format: Journal Article
• Language: English
• Published: United States Endocrine Society 01-04-2011; Oxford University Press
• Subjects: Animals; Anti-Mullerian Hormone - genetics; Biochemistry, Molecular Biology; Cells, Cultured; Cyclic AMP - metabolism; Cyclic AMP-Dependent Protein Kinases - metabolism; Female; Follicle Stimulating Hormone - metabolism; GATA4 Transcription Factor - metabolism; Genes, Reporter; Gonadotropins - metabolism; Granulosa Cells - metabolism; Humans; Life Sciences; Luteinizing Hormone - metabolism; Mice; Original Research; Ovarian Follicle - metabolism; p38 Mitogen-Activated Protein Kinases - metabolism; Reproductive Biology; Reverse Transcriptase Polymerase Chain Reaction; Sexual reproduction; Steroidogenic Factor 1 - metabolism; Transcription, Genetic
• ISSN: 0888-8809; 1944-9917
• DOI: 10.1210/me.2010-0297

Follicle Stimulating Hormone and cyclic adenosine 5′-monophosphate enhance the transcription of anti-Müllerian hormone both in human granulosa-luteal cells and in the KK1 granulosa cell line.
Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance, a member of the TGF-ß family, is responsible for the regression of Müllerian ducts in the male fetus. In females, AMH is synthesized by granulosa cells of preantral and small antral follicles, and production wanes at later stages of follicle maturation. Using RT-PCR in luteal granulosa cells in primary culture and reporter gene techniques in the KK1 granulosa cell line, we show that FSH and cAMP enhance AMH transcription, and LH has an additive effect. Gonadotropins and cAMP act through protein kinase A and p38 MAPK signaling pathways and involve the GATA binding factor-4 and steroidogenic factor-1 transcription factors, among others. The expression profile of AMH and the dynamics of serum AMH after gonadotropin stimulation have been interpreted as a down-regulating effect of FSH upon AMH production by granulosa cells. The specific effect of gonadotropins upon granulosa cells may be obscured in vivo by the effect of FSH upon follicular maturation and by the presence of other hormones and growth factors, acting individually or in concert.