Demarcating the gene-rich regions of the wheat genome
By physically mapping 3025 loci including 252 phenotypically characterized genes and 17 quantitative trait loci (QTLs) relative to 334 deletion breakpoints, we localized the gene-containing fraction to 29% of the wheat genome present as 18 major and 30 minor gene-rich regions (GRRs). The GRRs varied...
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| Published in: | Nucleic acids research Vol. 32; no. 12; pp. 3546 - 3565 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
England
Oxford University Press
2004
Oxford Publishing Limited (England) |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | By physically mapping 3025 loci including 252 phenotypically characterized genes and 17 quantitative trait loci (QTLs) relative to 334 deletion breakpoints, we localized the gene-containing fraction to 29% of the wheat genome present as 18 major and 30 minor gene-rich regions (GRRs). The GRRs varied both in gene number and density. The five largest GRRs physically spanning <3% of the genome contained 26% of the wheat genes. Approximate size of the GRRs ranged from 3 to 71 Mb. Recombination mainly occurred in the GRRs. Various GRRs varied as much as 128-fold for gene density and 140-fold for recombination rates. Except for a general suppression in 25–40% of the chromosomal region around centromeres, no correlation of recombination was observed with the gene density, the size, or chromosomal location of GRRs. More than 30% of the wheat genes are in recombination-poor regions thus are inaccessible to map-based cloning. |
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| Bibliography: | http://www.nar.oupjournals.org/ Received January 14, 2004; Revised March 28, 2004; Accepted May 14, 2004 To whom correspondence should be addressed at Department of Crop and Soil Sciences, 277 Johnson Hall, PO Box 646420, Washington State University, Pullman, WA 99164, USA. Tel: +509 335 4666; Fax: +509 335 8674; Email: ksgill@wsu.edu Present addresses: Devinder Sandhu, G302 Agronomy Hall, Department of Agronomy, Iowa State University, Ames, IA-50011-1010, USA Mustafa Erayman, Agricultural College, Department of Crop Sciences, Mustafa Kemal University, 31034 Hatay, Turkey Muharrem Dilbirligi, Central Research Institute for Field Crops, Eskisehir yolu, 10 km, Lodumulu/Ankara, Pk: 226, 0642 Ulus/Ankara, Turkey The authors wish it to be known that, in their opinion, the first four authors should be regarded as joint First Authors local:gkh639 ark:/67375/HXZ-TPRS9CHZ-J istex:70188E69EEF36AB45C7D30EABEE1671EBCAD6E7F ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Present address: Mustafa Erayman, Agricultural College, Department of Crop Sciences, Mustafa Kemal University, 31034 Hatay, Turkey The authors wish it to be known that, in their opinion, the first four authors should be regarded as joint First Authors To whom correspondence should be addressed at Department of Crop and Soil Sciences, 277 Johnson Hall, PO Box 646420, Washington State University, Pullman, WA 99164, USA. Tel: +509 335 4666; Fax: +509 335 8674; Email: ksgill@wsu.edu Present address: Muharrem Dilbirligi, Central Research Institute for Field Crops, Eskisehir yolu, 10 km, Lodumulu/Ankara, Pk: 226, 0642 Ulus/Ankara, Turkey Present address: Devinder Sandhu, G302 Agronomy Hall, Department of Agronomy, Iowa State University, Ames, IA-50011-1010, USA |
| ISSN: | 0305-1048 1362-4962 1362-4962 |
| DOI: | 10.1093/nar/gkh639 |