Differential feedback regulation of ethylene biosynthesis in pulp and peel tissues of banana fruit

• Published in: Journal of experimental botany Vol. 58; no. 5; pp. 1047 - 1057
• Main Authors: Inaba, Akitsugu, Liu, Xuejun, Yokotani, Naoki, Yamane, Miki, Lu, Wang-Jin, Nakano, Ryohei, Kubo, Yasutaka
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-01-2007; Oxford Publishing Limited (England)
• Subjects: Agronomy. Soil science and plant productions; Amino Acids, Cyclic - metabolism; Banana fruit; Biological and medical sciences; Biosynthesis; Climacteric; Complementary DNA; Cyclopropanes - pharmacology; Economic plant physiology; enzyme activity; ethylene biosynthesis; Ethylene production; Ethylenes - biosynthesis; Fructification, ripening. Postharvest physiology; Fruit - metabolism; fruit ripening; Fundamental and applied biological sciences. Psychology; gene expression; Gene expression regulation; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - physiology; Genes; Growth and development; Lyases - metabolism; Messenger RNA; Molecular Sequence Data; Musa - drug effects; Musa - metabolism; Peels; Plant Proteins - genetics; Plant Proteins - metabolism; Propylene; pulp and peel tissue; Research Papers; Ripening; Time Factors; ethylene biosynthesis; fruit ripening; pulp and peel tissue; Banana fruit; enzyme activity; gene expression; Monocotyledones; Fruit; Ethylene; Musaceae; Biosynthesis; Gene expression; Ripening; Musa; Angiospermae; Spermatophyta
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/erl265

The feedback regulation of ethylene biosynthesis in banana [Musa sp. (AAA group, Cavendish subgroup) cv. Grand Nain] fruit was investigated in an attempt to clarify the opposite effect of 1-methylcyclopropene (1-MCP), an ethylene action inhibitor, before and after the onset of ripening. 1-MCP pre-treatment completely prevented the ripening-induced effect of propylene in pre-climacteric banana fruit, whereas treatment after the onset of ripening stimulated ethylene production. In pre-climacteric fruit, higher concentrations of propylene suppressed ethylene production more strongly, despite their earlier ethylene-inducing effect. Exposure of the fruit ripened by propylene to 1-MCP increased ethylene production concomitantly with an increase in 1-aminocyclopropane-1-carboxylate (ACC) synthase activity and ACC content, and prevented a transient decrease in MA-ACS1 transcripts in the pulp tissues. In contrast, in the peel of ripening fruit, 1-MCP prevented the increase in ethylene production and subsequently the ripening process by reduction of the increase in MA-ACS1 and MA-ACO1 transcripts and of ACC synthase and ACC oxidase activities. These results suggest that ethylene biosynthesis in ripening banana fruit may be controlled negatively in the pulp tissue and positively in the peel tissue. This differential regulation by ethylene in pulp and peel tissues was also observed for MA-PL, MA-Exp, and MA-MADS genes.