Hydrogen-limited growth of hyperthermophilic methanogens at deep-sea hydrothermal vents

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 109; no. 34; pp. 13674 - 13679
• Main Authors: Ver Eecke, Helene C, Butterfield, David A, Huber, Julie A, Lilley, Marvin D, Olson, Eric J, Roe, Kevin K, Evans, Leigh J, Merkel, Alexandr Y, Cantin, Holly V, Holden, James F
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 21-08-2012; National Acad Sciences
• Subjects: Archaea - growth & development; Archaea - physiology; Bacteria; Biodiversity; Biogeochemistry; Biological Sciences; bioreactors; Chimneys; coculture; Coculture Techniques; DNA, Ribosomal - metabolism; Ecosystem; Enzyme kinetics; Fluids; Gases; Genes; Geography; Heterotrophs; Hydrogen; Hydrogen - chemistry; Hydrothermal Vents; Kinetics; metabolism; Methane; Methane - chemistry; methane production; Methanocaldococcus jannaschii; Methanogens; microbial growth; Molecular Sequence Data; Ocean bottom; Oceans; Submarine hydrothermal vents; Temperature; Time Factors; Volcanoes; Water Microbiology; Pacific Ocean
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1206632109

Microbial productivity at hydrothermal vents is among the highest found anywhere in the deep ocean, but constraints on microbial growth and metabolism at vents are lacking. We used a combination of cultivation, molecular, and geochemical tools to verify pure culture H ₂ threshold measurements for hyperthermophilic methanogenesis in low-temperature hydrothermal fluids from Axial Volcano and Endeavour Segment in the northeastern Pacific Ocean. Two Methanocaldococcus strains from Axial and Methanocaldococcus jannaschii showed similar Monod growth kinetics when grown in a bioreactor at varying H ₂ concentrations. Their H ₂ half-saturation value was 66 μM, and growth ceased below 17–23 μM H ₂, 10-fold lower than previously predicted. By comparison, measured H ₂ and CH ₄ concentrations in fluids suggest that there was generally sufficient H ₂ for Methanocaldococcus growth at Axial but not at Endeavour. Fluids from one vent at Axial (Marker 113) had anomalously high CH ₄ concentrations and contained various thermal classes of methanogens based on cultivation and mcrA / mrtA analyses. At Endeavour, methanogens were largely undetectable in fluid samples based on cultivation and molecular screens, although abundances of hyperthermophilic heterotrophs were relatively high. Where present, Methanocaldococcus genes were the predominant mcrA / mrtA sequences recovered and comprised ∼0.2–6% of the total archaeal community. Field and coculture data suggest that H ₂ limitation may be partly ameliorated by H ₂ syntrophy with hyperthermophilic heterotrophs. These data support our estimated H ₂ threshold for hyperthermophilic methanogenesis at vents and highlight the need for coupled laboratory and field measurements to constrain microbial distribution and biogeochemical impacts in the deep sea.