Transcriptome analysis of resistant and susceptible genotypes of Glycine tomentella during Phakopsora pachyrhizi infection reveals novel rust resistance genes

• Published in: Theoretical and applied genetics Vol. 120; no. 7; pp. 1315 - 1333
• Main Authors: Soria-Guerra, Ruth Elena, Rosales-Mendoza, Sergio, Chang, Sungyul, Haudenshield, James S, Padmanaban, Annamalai, Rodriguez-Zas, Sandra, Hartman, Glen L, Ghabrial, Said A, Korban, Schuyler S
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-05-2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agriculture; Basidiomycota - physiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cell cycle; Classical genetics, quantitative genetics, hybrids; disease resistance; Disease Susceptibility; Diseases and pests; Down-Regulation - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; gene expression regulation; Gene Expression Regulation, Plant; Genes; Genes, Plant - genetics; Genetic aspects; Genetic engineering; Genetics of eukaryotes. Biological and molecular evolution; Genotype; Glycine max - cytology; Glycine max - genetics; Glycine max - immunology; Glycine max - microbiology; Glycine tomentella; Health aspects; host plants; Immunity, Innate - genetics; Infections; Life Sciences; Metabolism; Original Paper; Pathogens; Phakopsora pachyrhizi; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; plant pathogenic fungi; Plant resistance; Polymerase chain reaction; Proteins; Pteridophyta, spermatophyta; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA; rust diseases; Soybean; Soybeans; transcriptome; Up-Regulation - genetics; Vegetals; United States > US; Illinois; WRKY Transcription Factor; Chalcone Synthases; Rust Resistance; Rust Infection; Rust Resistance Gene; Fungi; Infection; Phakopsora pachyrhizi; Resistance gene; Transcriptome; Genotype; Genetics; Agriculture; Glycine; Basidiomycota
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-009-1258-0

Soybean rust, caused by Phakopsora pachyrhizi, is a destructive foliar disease in nearly all soybean-producing countries. To identify genes controlling resistance to soybean rust, transcriptome profiling was conducted in resistant and susceptible Glycine tomentella genotypes triggered by P. pachyrhizi infection. Among 38,400 genes monitored using a soybean microarray, at 5% false discovery rate, 1,342 genes were identified exhibiting significant differential expression between uninfected and P. pachyrhizi-infected leaves at 12, 24, 48, and 72 h post-inoculation (hpi) in both rust-susceptible and rust-resistant genotypes. Differentially expressed genes were grouped into 12 functional categories, and among those, large numbers relate to basic plant metabolism. Transcripts for genes involved in the phenylpropanoid pathway were up-regulated early during rust infection. Similarly, genes coding for proteins related to stress and defense responses such as glutathione-S-transferases, peroxidases, heat shock proteins, and lipoxygenases were consistently up-regulated following infection at all four time points. Whereas, subsets of genes involved in cellular transport, cellular communication, cell cycle, and DNA processing were down-regulated. Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) on randomly selected genes from the different categories confirmed these findings. Of differentially expressed genes, those associated with the flavonoid biosynthesis pathway as well as those coding for peroxidases and lipoxygenases were likely to be involved in rust resistance in soybean, and would serve as good candidates for functional studies. These findings provided insights into mechanisms underlying resistance and general activation of plant defense pathways in response to rust infection.