COL1A1-PDGFB Fusion Transcripts in Fibrosarcomatous Areas of Six Dermatofibrosarcomas Protuberans

• Published in: The Journal of molecular diagnostics : JMD Vol. 2; no. 1; pp. 47 - 52
• Main Authors: Wang, Jian, Morimitsu, Yosuke, Okamoto, Sumika, Hisaoka, Masanori, Ishida, Tsuyoshi, Sheng, Weiqi, Hashimoto, Hiroshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-02-2000; ASIP; American Society for Investigative Pathology
• Subjects: Adolescent; Adult; Aged; Artificial Gene Fusion; Base Sequence; Collagen - genetics; Collagen Type I; Dermatofibrosarcoma - genetics; Dermatofibrosarcoma - pathology; DNA Primers - genetics; DNA, Neoplasm - genetics; Female; Genes, sis; Humans; Male; Middle Aged; Regular; Reverse Transcriptase Polymerase Chain Reaction; Skin Neoplasms - genetics; Skin Neoplasms - pathology
• ISSN: 1525-1578; 1943-7811
• DOI: 10.1016/S1525-1578(10)60614-9

The fibrosarcomatous transformation of dermatofibrosarcoma protuberans (DFSP) has been considered for some time to be associated with an adverse clinical outcome. However, the molecular and cellular mechanism underlying the tumor progression remains undetermined. As the chimeric gene,
COL1A1-PDGFB, has been proposed to play an important role in the histogenesis of DFSP, we conducted a reverse transcription-polymerase chain reaction assay to ascertain whether the
COL1A1-PDGFB fusion transcripts can be detected in both conventional DFSP and fibrosarcomatous components of DFSP with fibrosarcomatous areas (DFSP-FS), using a simple method of microdissection on sections of archival formalin-fixed, paraffin-embedded tumor specimens from six DFSP-FS cases. The
COL1A1-PDGFB fusion transcripts could be detected in FS areas in five of the six cases, whereas conventional DFSP areas of all cases expressed the chimeric mRNA. A subsequent sequence analysis of the polymerase chain reaction products confirmed that the detected messages were derived from identical gene fusions in the two different components of each of the five cases. Our results verify that the
COL1A1-PDGFB fusion transcripts are preserved in the FS areas of most DFSP-FSs. The expression of the fusion transcripts in both conventional DFSP and FS areas of DFSP-FS supports a common histogenesis of the two components.