Isozyme application for variety identification and progeny hybridity in Japanese sugarcane [genus Saccharum]

• Published in: Breeding Science Vol. 49; no. 2; pp. 89 - 95
• Main Authors: Shoda, M. (Okinawa-ken. Agricultural Experiment Station, Naha (Japan)), Nagamine, T, Terauchi, T, Akamine, F, Sugimoto, A
• Format: Journal Article
• Language: English
• Published: Tokyo Japanese Society of Breeding 1999; Japan Science and Technology Agency
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; breeding; EPREUVE SUR LA DESCENDANCE; ESPECE; ESPECIES; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics and breeding of economic plants; HIBRIDACION; HYBRIDATION; HYBRIDIZATION; IDENTIFICACION; IDENTIFICATION; ISOENZIMAS; ISOENZYME; ISOENZYMES; METHODE D'ESSAI; METODOS DE ENSAYO; Plant material; PROGENY TESTING; PRUEBAS DE DESCENDENCIA; SACCHARUM; SPECIES; TRIAL METHODS; VARIEDADES; VARIETE; VARIETIES; Asia; Japan; Far east; Saccharum; Hybrid variety; Monocotyledones; Genetic marker; Enzyme; Isozyme; Isocitrate dehydrogenase (NADP; ); Esterases; Alcohol dehydrogenase; Enzymatic activity; Peroxidases; Gramineae; Angiospermae; Genetic improvement; Hydrolases; Herbaceous plant; Spermatophyta; Specific identification; Peroxidase; Oxidoreductases; Intraspecific comparison; Polymorphism
• ISSN: 1344-7610; 1347-3735
• DOI: 10.1270/jsbbs.49.89

In order to apply isozymes as genetic markers in sugarcane breeding, the polymorphism of 24 Japanese varieties, including breeding materials, was analyzed using 12 enzyme systems. Of the enzymes examined, the activity of Alcohol dehydrogenase (ADH), Esterase (EST), Glutamate oxaloacetate transaminase (GOT), Isocitric dehydrogenase (IDH) and Peroxidase (POX) was clearly revealed and polymorphism was detected among the varieties. The 24 varieties could be identified by these five enzyme systems. In the selfed progenies of NiF8, Ni9 and RK86-129, large numbers of bands in EST and POX were segregated. Segregation patterns insome of the isozymes in EST and POX showed a monogenic segregation ratio of 3 : 1. However, others deviated from the monogenic segregation ratio. In a parentage test using isozymes, the hybridity of almost all the seedlings could be identified in 10 cross combinations. These results indicate that the isozyme technique is a useful tool for variety identification an