Flavonoid Accumulation Patterns of Transparent Testa Mutants of Arabidopsis

• Published in: Plant physiology (Bethesda) Vol. 126; no. 2; pp. 536 - 548
• Main Authors: Peer, Wendy Ann, Dana E. Brown, Tague, Brian W., Muday, Gloria K., Taiz, Lincoln, Murphy, Angus S.
• Format: Journal Article
• Language: English
• Published: Legacy CDMS American Society of Plant Physiologists 01-06-2001; American Society of Plant Biologists
• Subjects: Accumulation; aglycone flavonols; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Auxins; Biological and medical sciences; Biosynthesis; Development and Hormone Action; Epidermis; Esters; Flavonoids; Flavonoids - metabolism; Flavonols; Fluorescence; Fluorescent Dyes - metabolism; Fundamental and applied biological sciences. Psychology; Growth regulators; Inflorescences; kaempferol; Kinetics; Life Sciences (General); Metabolism; Mutation; Plant physiology and development; Plant roots; Plant Roots - growth & development; Plants; Pollen; Root tips; Seedlings; Subcellular Fractions - metabolism; transparent testa (tt4) gene; Nasa Discipline Plant Biology; Non-Nasa Center; Metabolic intermediate; Flavanol; Auxin; Growth; Kaempferol; Plant juvenile growth stage; Biological transport; Genetic variant; Aglycone; Functional deficit; Biosynthesis; Flavonoid; Arabidopsis thaliana; Regulation(control); Cruciferae; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Intraspecific comparison; Experimental plant; Biological accumulation; Localization; Histochemistry; NASA Discipline Plant Biology; Non-NASA Center
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.126.2.536

Flavonoids have been implicated in the regulation of auxin movements in Arabidopsis. To understand when and where flavonoids may be acting to control auxin movement, the flavonoid accumulation pattern was examined in young seedlings and mature tissues of wild-type Arabidopsis. Using a variety of biochemical and visualization techniques, flavonoid accumulation in mature plants was localized in cauline leaves, pollen, stigmata, and floral primordia, and in the stems of young, actively growing inflorescences. In young Landsberg erecta seedlings, aglycone flavonols accumulated developmentally in three regions, the cotyledonary node, the hypocotyl-root transition zone, and the root tip. Aglycone flavonols accumulated at the hypocotyl-root transition zone in a developmental and tissue-specific manner with kaempferol in the epidermis and quercetin in the cortex. Quercetin localized subcellularly in the nuclear region, plasma membrane, and endomembrane system, whereas kaempferol localized in the nuclear region and plasma membrane. The flavonoid accumulation pattern was also examined in transparent testa mutants blocked at different steps in the flavonoid biosynthesis pathway. The transparent testa mutants were shown to have precursor accumulation patterns similar to those of end product flavonoids in wild-type Landsberg erecta, suggesting that synthesis and end product accumulation occur in the same cells.