effect of pretreatment or combined treatment of quercetin on menadione toxicity in rat primary mixed glial cells in vitro

• Published in: Cytotechnology (Dordrecht) Vol. 61; no. 1-2; pp. 11 - 16
• Main Authors: Oztopcu-Vatan, Pinar, Kabadere, Selda, Uyar, Ruhi
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01-11-2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Antioxidants; Biochemistry; Biological and medical sciences; Biomedicine; Biotechnology; Cell culture; Cells; Chemistry; Chemistry and Materials Science; Cytotoxicity; Drug dosages; Food plants; Fundamental and applied biological sciences. Psychology; Glial cells; Menadione; Neurodegenerative diseases; Neuronal-glial interactions; Original Research; Oxidative stress; Quercetin; Reactive oxygen species; Toxicity; Vitamin K; Menadione; Glia; MTT; In vitro; Quercetin; Glial cell; Rat; Toxicity; Rodentia; Vertebrata; Mammalia; Treatment; Pretreatment
• ISSN: 0920-9069; 1573-0778
• DOI: 10.1007/s10616-009-9235-7

Neurons and glia are highly susceptible to reactive oxygen species that play a key role in various neurodegenerative diseases. Menadione, a synthetic derivative of vitamin K, induces reactive oxygen generation. Quercetin one of the most ubiquitous bioflavonoids in food of plant origin, has strong antioxidant activities on different cell types, however recent studies demonstrated that it has also prooxidant and cytotoxic potentials. We examined the action of pre- and co-treatment of quercetin on menadione induced glial toxicity. The primary mixed glial cells obtained from 1 to 3 day old rat brain were pretreated with 10, 25, 100 or 250 μM quercetin for 1 h, washed out and 10, 25, 50, 75 or 100 μM menadione was added for 6 h. The other group of cells was treated with respective doses of quercetin combined simultaneously with the same doses of menadione for 6 h. The cells were washed and incubated for additional 24 h for recovery period and the viability was measured by using MTT assay. Menadione was dose-dependently toxic to glia cells and pretreatment with respective quercetin doses for 1 h could not eliminate this toxicity. Although 10 and 25 μM quercetin combined with 10 and 25 μM menadione could not change, 100 and 250 μM quercetin together with 10 or 25 μM menadione for 6 h increased further the menadione induced toxicity. We conclude that when combined with menadione, quercetin at high doses could be toxic to primary rat glia cells in culture.