Comparative mapping of bovine chromosome 13 by fluorescence in situ hybridization

• Published in: Animal genetics Vol. 29; no. 4; pp. 265 - 272
• Main Authors: Schläpfer, J., Gallagher Jr, D. S., Burzlaff, J. D., Womack, J. E., Stelly, D. M., Taylor, J. F., Davis, S. K.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-08-1998; Blackwell
• Subjects: Animals; Biological and medical sciences; bovine; bovine bacterial artificial chromosome library; cattle; Cattle - genetics; Chromosome Banding - veterinary; Chromosome Mapping - veterinary; chromosomes; Chromosomes, Bacterial; Classical genetics, quantitative genetics, hybrids; DNA Primers; fluorescence in situ hybridization; Fundamental and applied biological sciences. Psychology; Gene Library; Genetics of eukaryotes. Biological and molecular evolution; In Situ Hybridization, Fluorescence - veterinary; physical gene mapping; Vertebrata; Genetic mapping; Vertebrata; Mammalia; Bovine; Gene; Genetic marker; Synteny; Chromosome 13; Artiodactyla; Ungulata; Physical map
• ISSN: 0268-9146; 1365-2052
• DOI: 10.1046/j.1365-2052.1998.00321.x

We present chromosomal fluorescence in situ hybridization (FISH) results that both extend the HSA20/BTA13 comparative map as well as cytogenetically anchor two microsatellite markers. A bovine bacterial artificial chromosome (BAC) library was screened for conserved genes (type I loci) previously assigned to HSA10 or HSA20 and BTA13, and for microsatellites selected from two published BTA13 linkage maps. Clones from six out of nine comparative loci and both microsatellites were found represented in the BAC library. These BAC clones were used as probes in single colour FISH to determine the chromosome band position of each locus. As predicted by the human/bovine comparative map, all type I loci mapped to BTA13. Because single colour FISH analysis revealed that the loci were clustered within the distal half of BTA13, dual colour FISH was used to confirm the locus order. Established order was centromere‐PRNP‐(SODIL/AVP/OXT)‐(BL42/GNAS1)‐HCK‐CSSM30. The findings confirm the presence of a conserved HSA20 homologous synteny group on BTA13 distal of a HSA10 homologous segment.