Derivation of human embryonic stem cell lines from embryos obtained after IVF and after PGD for monogenic disorders

• Published in: Human reproduction (Oxford) Vol. 21; no. 2; pp. 503 - 511
• Main Authors: Mateizel, I., De Temmerman, N., Ullmann, U., Cauffman, G., Sermon, K., Van de Velde, H., De Rycke, M., Degreef, E., Devroey, P., Liebaers, I., Van Steirteghem, A.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-02-2006; Oxford Publishing Limited (England)
• Subjects: Biological and medical sciences; Biomarkers; Cell Culture Techniques; Cell Differentiation; Cell Line; cystic fibrosis; Cystic Fibrosis - diagnosis; Cystic Fibrosis - genetics; disease models; Embryo, Mammalian - cytology; Female; Fertilization in Vitro; Genetic Diseases, Inborn - diagnosis; Gynecology. Andrology. Obstetrics; human embryonic stem cells; Humans; Huntington disease; Huntington Disease - diagnosis; Huntington Disease - genetics; Medical sciences; myotonic dystrophy; Myotonic Dystrophy - diagnosis; Myotonic Dystrophy - genetics; Pluripotent Stem Cells - cytology; Pregnancy; Preimplantation Diagnosis; myotonic dystrophy; disease models; cystic fibrosis; human embryonic stem cells; Huntington disease; Embryo; Stem cell; Assisted procreation; Genetics; Degenerative disease; Pancreatic disease; Human; Neuromuscular diseases; Nervous system diseases; Respiratory disease; Myotonia; cystic fibrosis/disease models/human embryonic stem cells/Huntington disease/myotonic dystrophy; Metabolic diseases; Cystic fibrosis; Cerebral disorder; Genetic disease; In vitro fertilization embryo transfer; Vertebrata; Cell line; Mammalia; Myotonic dystrophy; Central nervous system disease; Digestive diseases; Models; Extrapyramidal syndrome
• ISSN: 0268-1161; 1460-2350
• DOI: 10.1093/humrep/dei345

BACKGROUND: Human embryonic stem (hES) cells are pluripotent cells usually derived from the inner cell mass (ICM) of blastocysts. Because of their ability to differentiate into all three embryonic germ layers, hES cells represent an important material for studying developmental biology and cell replacement therapy. hES cell lines derived from blastocysts diagnosed as carrying a genetic disorder after PGD represent in vitro disease models. METHODS: ICMs isolated by immunosurgery from human blastocysts donated for research after IVF cycles and after PGD were plated in serum-free medium (except VUB01) on mouse feeder layers. RESULTS: Five hES cell lines were isolated, two from IVF embryos and three from PGD embryos. All lines behave similarly in culture and present a normal karyotype. The lines express all the markers considered characteristic of undifferentiated hES cells and were proven to be pluripotent both in vitro and in vivo (ongoing for VUB05_HD). CONCLUSIONS: We report here on the derivation of two hES cell lines presumed to be genetically normal (VUB01 and VUB02) and three hES cell lines carrying mutations for myotonic dystrophy type 1 (VUB03_DM1), cystic fibrosis (VUB04_CF) and Huntington disease (VUB05_HD).