Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42

• Published in: Molecular plant-microbe interactions Vol. 20; no. 6; pp. 619 - 626
• Main Authors: Idris, E.E, Iglesias, D.J, Talon, M, Borriss, R
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01-06-2007; The American Phytopathological Society
• Subjects: Araceae - growth & development; Araceae - metabolism; Araceae - microbiology; Bacillus - classification; Bacillus - genetics; Bacillus - physiology; Bacillus amyloliquefaciens; biochemical pathways; Biological and medical sciences; Biological Assay; culture filtrates; Fundamental and applied biological sciences. Psychology; gas chromatography; Gas Chromatography-Mass Spectrometry; genes; Genes, Bacterial; high performance liquid chromatography; indole acetic acid; Indoleacetic Acids - analysis; Indoleacetic Acids - metabolism; mass spectrometry; microbial activity; Mutation - genetics; Phytopathology. Animal pests. Plant and forest protection; plant growth; plant growth-promoting rhizobacteria; secretion; Sequence Homology, Nucleic Acid; strain differences; strains; tryptophan; Tryptophan - biosynthesis; Tryptophan - metabolism; Tryptophan - pharmacology; Auxin; Microbiology; Enzyme; Secretion; Bacillus amyloliquefaciens; Bacillaceae; Metabolism; Strain; Gas chromatography; Plant; Bacillales; Gene; Indoleacetic acid; Bacteria; Hydrolases; Plant growth substance; Mutation; Phytohormone; Nitrilase
• ISSN: 0894-0282; 1943-7706
• DOI: 10.1094/mpmi-20-6-0619

Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (ΔtrpBA) and E102 (ΔtrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (ΔysnE, putative IAA transacetylase) and E105 (ΔyhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens.