Commonalities and Differences in Regulation of N-Acyl Homoserine Lactone Quorum Sensing in the Beneficial Plant-Associated Burkholderia Species Cluster

• Published in: Applied and Environmental Microbiology Vol. 76; no. 13; pp. 4302 - 4317
• Main Authors: Suárez-Moreno, Zulma Rocío, Devescovi, Giulia, Myers, Mike, Hallack, Letícia, Mendonça-Previato, Lucia, Caballero-Mellado, Jesús, Venturi, Vittorio
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-07-2010; American Society for Microbiology (ASM)
• Subjects: Acyl-Butyrolactones - metabolism; Acyl-Butyrolactones - pharmacology; Adaptations; Amino acids; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biofilms; Biological and medical sciences; Burkholderia - classification; Burkholderia - genetics; Burkholderia - growth & development; Burkholderia - metabolism; Burkholderia cepacia; Burkholderia glumae; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genetics and Molecular Biology; Genotype & phenotype; Gram-negative bacteria; Microbiology; Molecular Sequence Data; Oryza - microbiology; Proteins; Quorum Sensing - physiology; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Sequence Analysis, DNA; Species Specificity; Bacteria; Lactone; Regulation(control); Quorum sensing; Burkholderia
• ISSN: 0099-2240; 1098-5336; 1098-6596
• DOI: 10.1128/AEM.03086-09

The genus Burkholderia includes over 60 species isolated from a wide range of environmental niches and can be tentatively divided into two major species clusters. The first cluster includes pathogens such as Burkholderia glumae, B. pseudomallei, and B. mallei and 17 well-studied species of the Burkholderia cepacia complex. The other recently established cluster comprises at least 29 nonpathogenic species, which in most cases have been found to be associated with plants. It was previously established that Burkholderia kururiensis, a member of the latter cluster, possesses an N-acyl homoserine lactone (AHL) quorum-sensing (QS) system designated "BraI/R," which is found in all species of the plant-associated cluster. In the present study, two other BraI/R-like systems were characterized in B. xenovorans and B. unamae and were designated the BraI/RXEN and BraI/RUNA systems, respectively. Several phenotypes were analyzed, and it was determined that exopolysaccharide was positively regulated by the BraIR-like system in the species B. kururiensis, B. unamae, and B. xenovorans, highlighting commonality in targets. However, the three BraIR-like systems also revealed differences in targets since biofilm formation and plant colonization were differentially regulated. In addition, a second AHL QS system designated XenI2/R2 and an unpaired LuxR solo protein designated BxeR solo were also identified and characterized in B. xenovorans LB400T. The two AHL QS systems of B. xenovorans are not transcriptionally regulating each other, whereas BxeR solo negatively regulated xenI2. The XenI2/R2 and BxeR solo proteins are not widespread in the Burkholderia species cluster. In conclusion, the present study represents an extensive analysis of AHL QS in the Burkholderia plant-associated cluster demonstrating both commonalities and differences, probably reflecting environmental adaptations of the various species.